Library Subscription: Guest
International Journal of Medicinal Mushrooms

Published 12 issues per year

ISSN Print: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

Indexed in

Identification, Optimization of Culture Conditions, and Bioactive Potential of Chinese Caterpillar Mushroom Ophiocordyceps sinensis (Ascomycetes) Mycelium Isolated from Fruiting Body

Volume 21, Issue 9, 2019, pp. 931-942
DOI: 10.1615/IntJMedMushrooms.2019031841
Get accessGet access

ABSTRACT

The present study deals with the challenges acquainted with in vitro culture of Ophiocordyceps sinensis. We have optimized the culture conditions for the growth of O. sinensis mycelium in semi-synthetic liquid media and determined antibacterial potential of the cultured mycelia extracts. In this study, mycelia were isolated from fruiting bodies and the isolate was identified as O. sinensis anamorph based on sequencing of internal transcribed spacer region. We investigated different culture conditions to optimize the growth of mycelia. Through this investigation, the isolated strain was observed to have its optimum growth at temperature (20°C), which yielded biomass of 12.38 g/L and pH (6.0) yielded biomass of 11.24g/L. Further to augment the production of mycelia, different carbon and nitrogen sources were optimized for mycelium growth in liquid media, out of which sucrose and corn steep powder proved to be the best carbon and nitrogen sources yielding biomass 14.01 g/L and 14.14 g/L, respectively. The evaluation of aqueous and methanolic extracts for antibacterial activity depicted that these extracts are active against all bacterial strains tested here. Aqueous extract depicted minimum inhibitory concentration (MIC) of 0.312, 0.019, 0.078, 0.312, and 0.625 mg/mL and methanolic extract depicted 1.25, 0.078, 0.009, 1.25, and 0.156 mg/mL against Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes, respectively. These results led to optimization of enhanced biomass production of O. sinensis, which can be a better alternative approach for further physiological studies and large-scale cultivation of this mushroom for its utilization for therapeutics and nutraceutical values.

REFERENCES
  1. Seth R, Haider SZ, Mohan M. Pharmacology, phytochemistry and traditional uses of Cordyceps sinensis (Berk.) Sacc: a recent update for future prospects. Indian J Trad Know. 2014;13(3):551-56.

  2. Joshi RK. Phytochemical and medicinal aspects of Cordyceps sinensis (Berk.): A review. J Med Plants Stud. 2016;4(1):65-67.

  3. Yue K, Ye M, Zhou Z, Sun W, Lin X. The genus Cordyceps: a chemical and pharmacological review. J Pharm Pharmacol. 2013;65(4):474-93.

  4. Zhou X, Gong Z, Su Y, Lin J, Tang K. Cordyceps fungi: natural products, pharmacological functions and developmental products. J Pharm Pharmacol. 2009;61(3):279-91.

  5. Cainong D, Tao Y, Tiantian CA. A comparative study of antimicrobial, antioxidant and cytototoxic activities of methanol extracts from fruit bodies and fermented mycelia of caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes). Int J Med Mushrooms. 2014;16(5):485-95.

  6. Sapan KS, Nandini G, Narender SA. Evaluation of mycelia nutrients, bioactive compounds and antioxidants of five Himalayan entomopathogenic Ascomycetes fungi from India. Int J Med Mushrooms. 2015;17(7):661-69.

  7. Wai YC, Xue QW, Ka CS, Ang XS. Cosmetics and skincare benefits of cultivated mycelia from the Chinese caterpillar mushroom, Ophiocordyceps sinensis (Ascomycetes). Int J Med Mushrooms. 2018;20(7):623-36.

  8. Hobbs CH. Medicinal mushrooms: an exploration of tradition, healing and culture. Santa Cruz (CA): Botanica Press; 1995:251.

  9. Liu ZY, Yao YJ, Liang ZQ, Pegler DN, Chase MW. Molecular evidence for the anamorph-telemorph connection in Cordyceps sinensis. Mycol Res. 2001;105(7):827-32.

  10. Mamta, Mehrotra S, Amitabh, Kirar V, Vats P, Nandi SP, Negi PS, Mishra K. Phytochemical and antimicrobial activities of Himalayan Cordyceps sinensis. Indian J Exp Biol. 2015;53(1):36-43.

  11. Kuniyal CP, Sundriyal RC. Conservation salvage of Cordyceps sinensiscollectionin the Himalayan Mountains is neglected. Ecosyst Serv. 2013;3:40-43.

  12. Kai Y, Meng Y, Xiao L, Zuji Z. The artificial cultivation of medicinal caterpillar fungus, Ophiocordyceps sinensis (Ascomycetes): a review. Int J Med Mushrooms. 2013;15(5):425-34.

  13. Yu Y. Deep fermentation technology for Cordyceps sinensis Sacc. Chinese Patent. 1997;CN1036531C.

  14. Sung GH, Hywel-Jones NL, Sung JM, Luangsa-Ard JJ, Shrestha B, Spatafora JW. Phylogenetic classification of Cordyceps and clavicipitaceous fungi. Stud Mycol. 2007;57:5-59.

  15. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-74.

  16. AOAC. Official methods of analysis of AOAC International. 17th ed. Gaithersburg, MD: AOAC, 2000.

  17. Filipa SR, Lillian B, Ricardo CC, Ana C, Leo JLD, Van G, Marina S, Isabel CFRF. The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties. Food Chem Toxicol. 2013;62:91-98.

  18. Liu Z, Liang Z, Liu A, Yao Y, Yu Z. Molecular evidence for telemorph-anamorph connections in Cordyceps based on ITS-5.8S rDNA sequences. Mycol Res. 2002;106(9):1100-08.

  19. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proc Natl Acad Sci U S A. 2012;109(6):6241-46.

  20. Chen YQ, Wang N, Qu LH, Li TH, Zhang WM. Determination of the anamorph of Cordyceps sinensis inferred from the analysis of the ribosomal DNA internal transcribed spacers and 5.8S rDNA. Biochem Syst Ecol. 2001;29(6):597-607.

  21. Hsu TH, Shiao LH, Hsiea C, Chang DM. A comparison of chemical composition and bioactive ingredients of the Chinese medicinal mushroom DongChongXiaCao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis. Food Chem. 2002;78(8):463-69.

  22. Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111-20.

  23. Dong CH, Yao YJ. Nutritional requirements of mycelial growth of Cordyceps sinensisin submerged culture. J Appl Microbiol. 2005;99(3):483-92.

  24. Yun FK, Jian CL, Chien SL, Chen YC, Jan M, Chuan SL, Shun FT, David MO, Chia CL, Hsin CL, John DY. Isolation, culture and characterization of Hirsutella sinensis mycelium from caterpillar fungus fruiting body. PLoS ONE. 2017;e0168734.

  25. Amin SMR, Alam N, Tania M, Khan MA. Study of mycelia growth of Cordyceps sinensis in different media at different pH and temperature. Bangladesh J Mushrooms. 2008;2(2):43-48.

  26. Dong CH, Yao YJ. On the reliability of fungal materials used in the studies on Ophiocordyceps sinensis. J Ind Microbiol Biotechnol. 2011;38(8):1027-35.

  27. Dai L, Lan J. Producing process of Chinese caterpillar fungus hypha fermentation. 1994; Patent CN1095103A.

  28. Choi GS, Shin YS, Kim JE, Ye YM, Parks HS. Five cases of food allergy to vegetable worm (Cordyceps sinensis) showing cross reactivity with silkworm pupae. Allergy. 2010;65(9):1196-97.

  29. Chattopadhyay S, Patra R, Ramamurthy T, Chowdhury A, Santra A, Dhali G, Bhattacharya SK, Berg DE, Nair GB, Mukhopadhyay AK. PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens. J Clin Microbiol. 2004;42(6):2821-24.

CITED BY
  1. Singh Amanvir, Goswami Arkaja, Nain Sonia, Enhanced antibacterial activity and photo-remediation of toxic dyes using Ag/SWCNT/PPy based nanocomposite with core–shell structure, Applied Nanoscience, 10, 7, 2020. Crossref

  2. Kaushik Vikas, Singh Amanvir, Arya Aditi, Sindhu Sangeeta Chahal, Sindhu Anil, Singh Ajay, Enhanced production of cordycepin in Ophiocordyceps sinensis using growth supplements under submerged conditions, Biotechnology Reports, 28, 2020. Crossref

  3. Al-Obaidi Jameel R., Alobaidi Khalid H., Al-Taie Bilal Salim, Wee David Hong-Sheng, Hussain Hasnain, Jambari Nuzul Noorahya, Ahmad-Kamil E. I., Ariffin Nur Syamimi, Uncovering Prospective Role and Applications of Existing and New Nutraceuticals from Bacterial, Fungal, Algal and Cyanobacterial, and Plant Sources, Sustainability, 13, 7, 2021. Crossref

  4. Kityania Sibashish, Nath Rajat, Das Subrata, Nath Deepa, Patra Jayanta Kumar, Talukdar Anupam Das, Fungi as Nutraceutical: Present to Future, in Applied Mycology, 2022. Crossref

2819 Article views 41 Article downloads Metrics
2819 VIEWS 41 DOWNLOADS 4 Crossref CITATIONS Google
Scholar
CITATIONS

Articles with similar content:

Antioxidant Potential of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) Cultivated on Artocarpus heterophyllus Sawdust Substrate in India International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Uma Maheshwari, Merlin Rajesh Lal, P. Rani, Sreeram Krishnan
Antioxidant Properties of Triterpenoids Isolated from Bagasse-Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), at Different Developmental Stages International Journal of Medicinal Mushrooms, Vol.24, 2022, issue 7
Lining Wang, Qinghua Huang, Liulian Zhang, Qingfu Wang, Biao Hu, Lei Liang
Chemical Composition and Antioxidant Properties of Treptacantha baccata (Fucales, Ochrophyta) from the Atlantic Coast of Morocco International Journal on Algae, Vol.24, 2022, issue 2
K. Khaya , A. Reani , C. Katif , C. Jama , B. Sabour , A. Raja , Z. Belattmania , F. Bentiss
In Vitro Antitumor Activity and Structure Characterization of Ethanol Extracts from Wild and Cultivated Chaga Medicinal Mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae) International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 2
Xian-Hui Chen, Ting Yin, Rempel B. Curtis, Gong Zhang, Zhan-Hui Lu, Yong Sun, Ji-Hong Jiang
Random Mutagenesis and Genetic Diversity of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes), by Physical Mutagens International Journal of Medicinal Mushrooms, Vol.25, 2023, issue 1
Xu Zhao, Ying Gan, Jin Zhao, Dinghong Jia, Bingcheng Gan, Xing Han, Rencai Feng, Renyun Miao, Zhiyuan Zhang, Junbin Lin, Jamila Akter Tuly, Yating Dong, Junjie Yan, Haile Ma
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain