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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i2.20
pages 105-116

Effect of Selenium-Enriched Agaricus bisporus (Higher Basidiomycetes) Extracts, Obtained by Pressurized Water Extraction, on the Expression of Cholesterol Homeostasis Related Genes by Low-Density Array

Alicia Gil-Ramirez
Department of Production and Characterization of New Foods, CIAL-Research Institute in Food Science (UAM+CSIC), Campus de Cantoblanco, 28049 Madrid, Spain
Cristina Soler-Rivas
Department of Production and Characterization of Novel Foods, CIAL–Research Institute in Food Science, Universidad Autónoma de Madrid, Madrid, Spain
Arantxa Rodriguez-Casado
IMDEA Food Institute, Carretera de Cantoblanco, no. 8, Campus de Cantoblanco, 28049 Madrid, Spain
Alejandro Ruiz-Rodriguez
Department of Production and Characterization of New Foods, CIAL-Research Institute in Food Science (UAM+CSIC), Campus de Cantoblanco, 28049 Madrid, Spain
Guillermo Reglero
Department of Production and Characterization of New Foods, CIAL-Research Institute in Food Science (UAM+CSIC), Campus de Cantoblanco, 28049 Madrid, Spain; IMDEA Food Institute, Carretera de Cantoblanco, no. 8, Campus de Cantoblanco, 28049 Madrid
Francisco Ramon Marin
Department of Production and Characterization of New Foods, CIAL-Research Institute in Food Science (UAM+CSIC), Campus de Cantoblanco, 28049 Madrid, Spain

ABSTRACT

Culinary-medicinal mushrooms are able to lower blood cholesterol levels in animal models by different mechanisms. They might impair the endogenous cholesterol synthesis and exogenous cholesterol absorption during digestion. Mushroom extracts, obtained using pressurized water extractions (PWE) from Agaricus bisporus basidiomes, supplemented or not supplemented with selenium, were applied to HepG2 cell cultures to study the expression of 19 genes related to cholesterol homeostasis by low-density arrays (LDA). Only the PWE fractions obtained at 25°C showed 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) inhibitory activity. Besides the enzymatic inhibition, PWE extracts may downregulate some of the key genes involved in the cholesterol homeostasis, such as the squalene synthase gene (FDFT1), since its mRNA expression falls by one third of its initial value. In summary, A. bisporus extracts may also modulate biological cholesterol levels by molecular mechanisms further than the enzymatic way previously reported.


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