Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v16.i5.30
pages 431-447

Effects of Medium Components and Culture Conditions on Mycelial Biomass and the Production of Bioactive Ingredients in Submerged Culture of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus

Jian-Zhi Chen
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, Taiwan
Hui-Chen Lo
Department of Nutritional Science, Fu Jen Catholic University, #510, JhongZheng Rd., Xinzhuang District, New Taipei City, 24205, Taiwan, ROC
Fang-Yi Lin
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan
Shih-Liang Chang
School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan
Chang-Wei Hsieh
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.)
Zeng-Chin Liang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan
Wai-Jane Ho
Department of Medicinal Botanicals and Healthcare, Department of Bioresources, Da-Yeh University, Datsun, Changhua, 51591 Taiwan
Tai-Hao Hsu
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.); Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.); bDepartment of Food Science and Biotechnology, Da-Yeh University, Taiwan, R.O.C

ABSTRACT

The optimal culture conditions were investigated to maximize the production of mycelial biomass and bioactive ingredients in submerged cultivation of Xylaria nigripes, a Chinese medicinal fungus. The one-factor-at-a-time method was used to explore the effects of medium components, including carbon, nitrogen, mineral sources, and initial pH of the medium and environmental factors, such as culture temperature and rotation speed, on mycelial growth and production of bioactive ingredients. The results indicated that the optimal culture temperature and rotation speed were 25°C and 100 rpm in a medium with 20 g fructose, 6 g yeast extract, and 2 g magnesiun sulfate heptahydrate as carbon, nitrogen, and mineral sources, respectively, in 1 L distilled water with an initial medium pH of 5.5. With optimal medium components and conditions of cultivation, the maximal production of mycelial biomass was 6.64 ± 0.88 g/L, with maximal production of bioactive ingredients such as extracellular polysaccharides (2.36 ± 0.18 mg/mL), intracellular polysaccharides (2.38 ± 0.07 mg/g), adenosine (43.27 ± 2.37 mg/g), total polyphenols (36.57 ± 1.36 mg/g), and triterpenoids (31.29 ± 1.17 mg/g) in a shake flask culture. These results suggest that different bioactive ingredients including intracellular polysaccharides, adenosine, total polyphenols and triterpenoids in mycelia and extracellular polysaccharides in broth can be obtained from one simple medium for submerged cultivation of X. nigripes.


Articles with similar content:

Improvement on the Antioxidant Activity of Polysaccharides by Submerged Fermentation of Shiraia bambusicola GDMCC 60438 (Ascomycota)
International Journal of Medicinal Mushrooms, Vol.22, 2020, issue 6
You Luo, Xiaofei Tian, Zhenqiang Wu, Yuan Zhang
Production of Biomass, Polysaccharides, and Ganoderic Acid using Non-conventional Carbon Sources under Submerged Culture of the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 2
Diego F. Rojas, Paola A. Zapata, Lucia Atehortua
Production of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (Higher Basidiomycetes), Biomass and Polysaccharides by Solid State Cultivation
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 5
Vlatka Petravic-Tominac, Branka Wraber, Jozica Habijanic, Bojana Boh, Marin BEROVIC
Enhanced Bioactive Exopolysaccharide Production by Mossy Maze Polypore, Cerrena unicolor (Higher Basidiomycetes) in Submerged Culture Conditions
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 7
Bukay Yenice Gursu, Mustafa Yamac, Ayse Betul Karaduman, Burcu Altinay
Composition of Mycelia and Basidiomata of the Culinary-Medicinal Golden Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes) by Nuclear Magnetic Resonance Spectroscopy
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 10
Shin-Yi Lin, Carolyn M. Slupsky, Jeng-Leun Mau, Darya O. Mishchuk