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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019031922
pages 995-1006

Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)

Chun-Hsien Yang
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan
Chun-Han Su
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan
Shou-Chou Liu
Kang Jian Biotech Co., Ltd., Nantou, Taiwan
Lean-Teik Ng
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan


Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases. In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined. The results showed yields of CP and NDP of 3.42% and 1.17%, respectively. CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose. Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa). Furthermore, CP but not NDP had a triple helix conformation. The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages. From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.


  1. Weng SC, Chou CJ, Lin LC, Tsai WJ, Kuo YC. Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae. J Ethnopharmacol. 2002;83:79-85.

  2. Wang Q, Liu Z. Advances in studies on medicinal fungi Cordyceps cicadae. Chin Tradit Herbal Drugs. 2004;35:469-71.

  3. Zhu R, Chen YP, Deng YY, Zheng R, Zhong YF, Wang L, Du LP. Cordyceps cicadae extracts ameliorate renal malfunction in a remnant kidney model. J Zhejiang Univ Sci B. 2011;12:1024-33.

  4. Wang Q, Wang CL, He FG, Zhang HY. Research progress on native drug Isaria cicadae Miq. J Chin Oncol. 20i3;i9:227-30.

  5. Jin Z, Chen Y. Clinical observation on Cordyceps cicadae Shing Tang in preventing the progression of chronic renal failure. Chin Arch Tradit Chin Med. 2006;24:1457-59.

  6. Kim HS, Kim JY, Ryu HS, Shin BR, Kang JS, Kim HM, Kim YO, Hong JT, Kim Y, Han SB. Phenotypic and functional maturation of dendritic cells induced by polysaccharide isolated from Paecilomyces cicadae. J Med Food. 2011;14:847-56.

  7. Cai JF, Lu HY, Lu BZ. Research of anti-tumor effects of different purified components of Cordyceps cicadae in vitro. Chin Arch Tradit Chin Med. 2010;28:760-64.

  8. Sun Y, Wink M, Wang P, Lu H, Zhao H, Liu H, Wang S, Sun Y, Liang Z. Biological characteristics, bioactive components and antineoplastic properties of sporoderm-broken spores from wild Cordyceps cicadae. Phytomedicine. 2017;36:217-28.

  9. Wang H, Zhang J, Sit WH, Lee CY, Wan MF. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med. 2014;9:15. doi: 10.1186/1749-8546-9-15.

  10. Wang D, Wang J, Wang D, Yu X, Olatunji OJ, Ouyang Z, Wei Y. Neuroprotective effects of butanol fraction of Cordyceps cicadae on glutamate-induced damage in PC12 cells involving oxidative toxicity. Chem Biodivers. 2018;15:e1700385.

  11. Sharma SK, Gautam N. Chemical composition and antioxidant and antibacterial activities of cultured mycelia of four Clavi-cipitaceous mushrooms (Ascomycetes) from the Indian Himalayas. Int J Med Mushrooms. 2017;19:45-54.

  12. Hsu JH, Jhou BY, Yeh SH, Chen Yl, Chen CC. Healthcare functions of Cordyceps cicadae. J Nutr Food Sci. 2015;5:432. doi: 10.4172/2155-9600.1000432.

  13. Mizuno T. Medicinal effects and utilization of Cordyceps (Fr.) Link (Ascomycetes) and Isaria Fr. (mitosporic fungi) Chinese caterpillar fungi, "Tochukaso" (Review). Int J Med Mushrooms. 1999;3:251-61.

  14. Chen YL, Yeh SH, Lin TW, Chen CC, Chen CS, Kuo CF. A 90-day Subchronic toxicity study of submerged mycelial culture of Cordyceps cicadae (Ascomycetes) in rats. Int J Med Mushrooms. 2015;8:771-81.

  15. Giavasis I. Bioactive fungal polysaccharides as potential functional ingredients in food and nutraceuticals. Curr Opin Biotechnol. 2014;26:162-73.

  16. Wasser SP. Medicinal mushrooms in human clinical studies. Part I. anticancer, oncoimmunological, and immunomodulatory activities: a review. Int J Med Mushrooms. 2017;19:279-317.

  17. Su CH, Lai MN, Lin CC, Ng LT. Comparative characterization of physicochemical properties and bioactivities of polysaccharides from selected medicinal mushrooms. Appl Microbiol Biotechnol. 2016;100:4385-93.

  18. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350-56.

  19. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.

  20. Dai J, Wu Y, Chen SW, Zhu S, Yin HP, Wang M, Tang J. Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone. Carbo- hydr Polym. 2010;82:629-35.

  21. Ukai S, Matsuura S, Hara C, Kiho T, Hirose K. Structure of a new galactomannan from the ascocarps of Cordyceps cicadae Shing. Carbohydr Res. 1982;101:109-16.

  22. Kiho T, Miyamoto I, Nagai K, Ukai S, Hara C. A minor, protein-containing galactomannans from the insect-body portion of the fungal preparation Chan hua (Cordyceps cicadae). Carbohydr Res. 1988;181:207-15.

  23. Li SP, Li P, Dong TTX, Tsim KWK. Anti-oxidation activity of different types of natural Cordyceps sinensis and cultured Cordyceps mycelia. Phytomedicine. 2001;8:207-12.

  24. Li SP, Su ZR, Dong TTX, Tsim KWK. The fruiting body and its caterpillar host of Cordyceps sinensis show close resemblance in main constituents and anti-oxidation activity. Phytomedicine. 2002;9:319-24.

  25. Zhou X, Gong Z, Su Y, Lin J, Tang K. Cordyceps fungi: natural products, pharmacological functions and developmental products. J Pharm Pharmacol. 2009;61:279-91.

  26. Buranov AU, Mazza G. 2010. Extraction and characterization of hemicelluloses from flax shives by different methods. Carbohydr Polym. 2010;79:17-25.

  27. Barker SA, Bourne EJ, Stacey M, Whiffen DH. Infra-red spectra of carbohydrates. Part I. Some derivatives of D-glucopy-ranose. J Chem Soc. 1954;0:171-76.

  28. Lim SF, Zheng YM, Zou SW, Chen JP. Characterization of copper adsorption onto an alginate encapsulated magnetic sorbent by a combined FT-IR, XPS, and mathematical modeling study. Environ Sci Technol. 2008;42:2551-56.

  29. Ooi VEC, Liu F. A review of pharmacological activities of mushroom polysaccharides. Int J Med Mushrooms. 1999;1(3): 195-206.

  30. Wang XH, Xu XJ, Zhang LN. Thermally induced conformation transition of triple-helical lentinan in NaCl aqueous solution. J Phys Chem B. 2008;112:10343-51.

  31. Eigler A, Sinha B, Hartmann G, Endres S. Taming TNF: strategies to restrain this proinflammatory cytokine. Immunol Today. 1997;18:487-92.

  32. Xie QW, Whisnant R, Nathan C. Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon y and bacterial lipopolysaccharide. J Exp Med. 1993;177:1779-84.

  33. Adams V, Nehrhoff B, Spate U, Linke A, Schulze PC, Baur A, Gielen S, Hambrecht R, Schuler G. Induction of iNOS expression in skeletal muscle by IL-1P and NF-kB activation: an in vitro and in vivo study. Cardiovasc Res. 2002;54:95-104.

  34. Hart PH, Brand C, Carson CF, Riley TV, Prager RH, Finlay-Jones JJ. Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes. Inflamm Res. 2000;49:619-26.

  35. Juergens UR, Engelen J, Racke K, Stober M, Gillissen A, Vetter H. Inhibitory activity of 1,8-cineole (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes. Pulm Pharmacol Ther. 2004;17:281-87.