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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017024590
pages 1113-1121

Extraction Optimization, Composition Analysis, and Antioxidation Evaluation of Polysaccharides from White Jelly Mushroom, Tremella fuciformis (Tremellomycetes)

Yu Zou
College of Life Science, Dalian Nationalities University, Dalian, China
Xiyan Hou
College of Life Science, Dalian Nationalities University, Dalian, China

ABSTRACT

We investigated the sonication-assisted extraction (SAE) conditions of Tremella fuciformis polysaccharides. Single-factor experiments showed that the optimal sonication intensity was 6 W/cm2; the other SAE conditions were further optimized using response surface experimental design. A Box-Behnken experiment exhibited that the optimal SAE conditions were temperature, 85°C; ratio of liquid to solid, 46 mL/g; and sonication time, 2 hours. The maximal polysaccharide yield (8.95 g/100 g) was obtained under the optimal extraction conditions. Polysaccharides extracted by sonication or traditional hot water extraction (TWE) were further separated and purified using DEAE-Sepharose and Sephadex G-100 column chromatography. Polysaccharide fractions (SAE-1, SAE-2, TWE-1, and TWE-2) were obtained; their molecular weights were 9.85 × 104, 6.64 × 104, 4.75 × 105, and 2.81 × 105 Da, respectively. The total sugar content of SAE-1 and SAE-2 was lower and the protein and nucleic acid contents were higher than those of TWE-1 and TWE-2. In addition, SAE-1 and SAE-2 showed stronger Fe2+-chelating activities and superoxide radical scavenging activities than TWE-1 and TWE-2. This suggests that polysaccharides extracted by sonication possess high antioxidant activities.


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