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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020035810
pages 991-1000

A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity Purified from the Field Blewit Mushroom Lepista personata (Agaricomycetes)

Lijing Xu
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, P.R. China; Collaborative Innovation Center of Advancing Quality and Efficiency of Loess Plateau Edible Fungi, Taigu, P.R. China
Xueran Geng
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, P.R. China; Collaborative Innovation Center of Advancing Quality and Efficiency of Loess Plateau Edible Fungi, Taigu, P.R. China
Mingchang Chang
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, P.R. China; Collaborative Innovation Center of Advancing Quality and Efficiency of Loess Plateau Edible Fungi, Taigu, P.R. China
Junlong Meng
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, P.R. China; Collaborative Innovation Center of Advancing Quality and Efficiency of Loess Plateau Edible Fungi, Taigu, P.R. China
Hexiang Wang
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China
Tzi Bun Ng
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

ABSTRACT

A ribonuclease was purified from dry fruiting bodies of the wild edible mushroom Lepista personata (LPR) to 259-fold with a specific activity of 280 U/mg. The purification protocol involved ion-exchange chromatography on DEAE-cellulose, CM-cellulose and SP-sepharose, followed by size exclusion chromatography on Superdex 75. LPR is a homodimeric protein with a molecular weight of 27.8 kDa as determined by SDS-PAGE and by gel filtration. Three inner peptide sequences for LPR were obtained by LC-MS-MS analysis. It demonstrated the optimum pH of 4.0 and temperature optimum of 60°C. The specificity ribonuclease potencies order toward polyhomoribonucleotides was poly C > poly A > poly G > poly U. The ribonuclease inhibited HIV-1 reverse transcriptase with an IC50 of 0.53 μM.

REFERENCES

  1. Ardelt W, Ardelt B, Darzynkiewicz Z. Ribonucleases as potential modalities in anticancer therapy. Eur J Pharmacol. 2009;625(1-3):181-9.

  2. Fang EF, Ng TB. Ribonucleases of different origins with a wide spectrum of medicinal applications. Biochim Biophys Acta. 2011;1815(1):65-74.

  3. Purusharth RI, Klein F, Sulthana S, Jager S, Jagannadham MV, Evguenieva-Hackenberg E, Ray MK, Klug G. Exoribonuclease R interacts with endoribonuclease E and an RNA helicase in the psychrotrophic bacterium Pseudomonas syringae Lz4W. J Biol Chem. 2005;280(15):14572-8.

  4. Perederina A, Esakova O, Quan C, Khanova E, Krasilnikov AS. Eukaryotic ribonucleases P/MRP: The crystal structure of the P3 domain. EMBO J. 2010;29(4):761-9.

  5. Polyakov KM, Lebedev AA, Okorokov AL, Panov KI, Schulga AA, Pavlovsky AG, Karpeisky MY, Dodson GG. The structure of substrate-free microbial ribonuclease binase and of its complexes with 3'GMP and sulfate ions. Acta Crystallogr D Biol Crystallogr. 2002;58(5):744-50.

  6. Kawano S, Kakuta Y, Nakashima T, Kimura M. Crystal structures of the Nicotiana glutinosa ribonuclease NT in complex with nucleoside monophosphates. J Biochem. 2006;140(3):375-81.

  7. Liu Q, Chen H, Wang HX, Ng TB. Isolation and characterization of a ubiquitin-like ribonuclease from the cultured deep root mushroom, Oudemansiella radicata (higher Basidiomycetes). Int J Med Mushrooms. 2015;17(11):1037-45.

  8. Shruti G, Sukhdev S, Singh KS. Purification and characterization of an extracellular ribonuclease from a Bacillus sp. RNS3 (KX966412). Int J Biol Macromol. 2017;97:440-6.

  9. Yamamura T, Ueda M, Psarras K, Suwa T, Watanaabe Y, Kameyama N, Tanabe M, Imamura H, Kitajima M. Immunosuppressive and anticancer effect of a mammalian ribonuclease that targets high-affinity interleukin-2-receptors. Eur J Surg. 2002;168(1):49-54.

  10. Zwolinska M, Smolewski P. Onconase: A ribonuclease with antitumor activity. Postepy Hig Med Dosw. 2010;64(2):58-66.

  11. Begam M, Narwal S, Roy S, Kumar S, Lodha ML, Kapoor HC. An antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata. Biochem (Moscow). 2006;71(1):44-8.

  12. Zhang JJ, Li Y, Zhou T, Xu DP, Zhang P, Li S, Li HB. Bioactivities and health benefits of mushrooms mainly from China. Molecules. 2016;21(7):1-16.

  13. Zhu M, Xu L, Chen X, Ma Z, Wang H, Ng TB. A novel ribonuclease with HIV-1 reverse transcriptase inhibitory activity from the edible mushroom Hygrophorus russula. Appl Biochem Biotechnol. 2013;170(1):219-30.

  14. Zhang R, Tian GT, Zhao YC, Zhao LY, Wang HX, Gong Z, Ng TB. A novel ribonuclease with HIV-1 reverse transcriptase inhibitory activity purified from the fungus Ramaria formosa. J Basic Microb. 2015;55(2):269-75.

  15. Guan GP, Wang HX, Ng TB. A novel ribonuclease with antiproliferative activity from fresh fruiting bodies of the edible mushroom Hypsizygus marmoreus. Biochim Biophys Acta. 2007;1770(12):1593-7.

  16. Zhao S, Zhao YC, Li SH, Zhang GQ, Wang HX, Ng TB. An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica. J Microbiol Biotechnol. 2010;20(4):693-9.

  17. Islam T, Ganesan K, Xu B (Bruce). New insight into mycochemical profiles and antioxidant potential of edible and medicinal mushrooms: a review. Int J Med Mushrooms. 2019;21(3):237-51.

  18. Teplyakova TV, Kosogova TA. Antiviral effect of Agaricomycetes mushrooms (Review). Int J Med Mushrooms. 2016;18(5):375-86.

  19. Wasser SP. Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: A review. Int J Med Mushrooms. 2017;19(4):279-317.

  20. Chang ST, Wasser SP. Current and future research trends in agricultural and biomedical applications of medicinal mushrooms and mushroom products (review). Int J Med Mushrooms. 2018;20(12):1121-33.

  21. Wang HX, Ng TB. A ribonuclease from the wild mushroom Boletus griseus. Appl Microbiol Biotechnol. 2006;72(5):912-6.

  22. Xu LJ, Chen QJ, Wang HX, Zhang GQ. Purification and characterization of a ribonuclease from the wild edible mushroom Armillaria luteo-virens. Indian J Biochem Biophys. 2013;50(3):196-201.

  23. Laemmli UK. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature. 1970;227(5259):680-5.

  24. Wang HX, Ng TB. Isolation of a new ribonuclease from fresh fruiting bodies of the straw mushroom. Biochem Biophys Res Commun. 1999;264:714-8.

  25. Zhao YC, Zhang GQ, Ng TB, Wang HX. A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune. J Microbiol. 2011;49(5):803-8.

  26. Wang HX, Ng TB. Purification and characterization of a potent homodimeric guanine-specific ribonuclease from fresh mushroom (Pleurotus tuber-regium) sclerotia. Int J Biochem Cell Biol. 2001;33(5):483-90.

  27. Ye XY, Ng TB. A novel and potent ribonuclease from fruiting bodies of the mushroom Pleurotus pulmonarius. Biochem Biophys Res Commun. 2002;293(2):857-61.

  28. Wang HX, Ng TB, Chiu SW. A distinctive ribonuclease from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum. Biochem Biophys Res Commun. 2004;314(2):519-22.

  29. Xiao C, Feng SS, Wang HX, Gong ZY, Ng TB. Purification and characterization of a ribonuclease with antiproliferative activity from the mystical wild mushroom Tuber indicum. J Basic Microb. 2014;54(1):102-8.

  30. Dan X, Liu W, Wong JH, Ng TB. A ribonuclease isolated from wild Ganoderma lucidum suppressed autophagy and triggered apoptosis in colorectal cancer cells. Front Pharmacol. 2016;7(6):1-13.

  31. Xia L, Chu KT, Ng TB. A low-molecular mass ribonuclease from the brown oyster mushroom. J Pept Res. 2005;66(1):1-8.

  32. Wang HX, Ngai HK, Ng TB. A ubiquitin-like peptide with ribonuclease activity against various polyhomoribonucleotides from the yellow mushroom Cantharellus cibarius. Peptides. 2003;24(4):509-13.

  33. Kobayashi H, Itagaki T, Inokuchi N, Ohgi K, Wada T, Iwama M, Irie M. A new type of RNase T2 ribonuclease in two Basidiomycetes fungi, Lentinus edodes and Irpex lacteus. Biosci Biotechnol Biochem. 2003;67(10):2307-10.

  34. Wu X, Zheng S, Cui L, Wang H, Ng TB. Isolation and characterization of a novel ribonuclease from the pink oyster mushroom Pleurotus djamor. J Gen Appl Microbiol. 2010;56(3):231-9.

  35. Wang HX, Ng TB. A novel ribonuclease from fresh fruiting bodies of the portabella mushroom Agaricus bisporus. Biochem Cell Biol. 2006;84(2):178-83.

  36. Wang HX, Ng TB. A ribonuclease with distinctive features from the wild green-headed mushroom Russulus virescens. Bio-chem Biophys Res Commun. 2003;312(4):965-8.


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