Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017021225
pages 685-696

Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Inhibits Candida Biofilms: A Metabolomic Approach

Anuja Bhardwaj
Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, Timarpur, Delhi, India
Payal Gupta
Department of Biotechnology, Graphic Era University, Dehradun, India
Navin Kumar
Department of Biotechnology, Graphic Era University, Dehradun, India
Jigni Mishra
Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, Timarpur, Delhi, India
Ajai Kumar
Advanced Instrumentation Research Facility, Jawaharlal Nehru University, New Delhi, India
Rakhee
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Kshipra Misra
Department of Biochemical Sciences (DBCS) Defense Institute of Physiology and Allied Sciences( DIPAS) Lucknow Road, Timarpur Delhi-110054

ABSTRACT

This article presents a comparative gas chromatography (GC)−mass spectrometry (MS)−based metabolomic analysis of mycelia and fruiting bodies of the medicinal mushroom Ganoderma lucidum. Three aqueous extracts−mycelia, fruiting bodies, and a mixture of them−and their sequential fractions (methanolic and ethyl acetate), prepared using an accelerated solvent extractor, were characterized by GC-MS to determine volatile organic compounds and by high-performance thin-layer chromatography to quantify ascorbic acid, a potent antioxidant. In addition, these extracts and fractions were assessed against Candida albicans and C. glabrata biofilms via the XTT reduction assay, and their antioxidant potential was evaluated. Application of chemometrics (hierarchical cluster analysis and principal component analysis) to GC data revealed variability in volatile organic compound profiles among G. lucidum extracts and fractions. The mycelial aqueous extract demonstrated higher anti-Candida activity and ascorbic acid content among all the extracts and fractions. Thus, this study illustrates the preventive effect of G. lucidum against C. albicans and C. glabrata biofilms along with its nutritional value.


Articles with similar content:

Antimicrobial Activity of Extracts of the Oyster Culinary Medicinal Mushroom Pleurotus ostreatus (Higher Basidiomycetes) and Identification of a New Antimicrobial Compound
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Ahmed M. Younis, Hussien H. El Shikh, Fang-Sheng Wu
Comparison of Antioxidant and Antiproliferation Activities of Polysaccharides from Eight Species of Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 3
Yangyang Yong, Yifan Gu, Zeliang Wang, Shizhu Zhang, Peiying Chen, Ling Lu
Comparison of the Composition and Antioxidant Activities of Phenolics from the Fruiting Bodies of Cultivated Asian Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 10
Peter Chi Keung Cheung, Shaoling Lin, Lai Tsz Ching, Xinxin Ke
Quantification of Water-Soluble Metabolites in Medicinal Mushrooms Using Proton NMR Spectroscopy
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 5
Carolyn M. Slupsky, Yu-Chang Lo, Jeng-Leun Mau, Shih-Chang Chien, Darya O. Mishchuk
Antibacterial Activity of Steroidal Compounds Isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetideae) Fruit Body
International Journal of Medicinal Mushrooms, Vol.1, 1999, issue 4
Rodrigo S. Cuneo, Artur Smania, Jr., Franco Delie Monache, Elza de Fatima Albino Smania