Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017021250
pages 709-716

Selective Cytotoxic Activity of Se-Methyl-Seleno-L-Cysteine– and Se-Polysaccharide–Containing Extracts from Shiitake Medicinal Mushroom, Lentinus edodes (Agaricomycetes)

Marzenna Klimaszewska
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland
Sandra Górska
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland
Maciej Dawidowski
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland
Piotr Podsadni
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland
Agnieszka Szczepanska
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland
Emilia Orzechowska
Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland; Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland
Dagmara Kurpios-Piec
Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
Emilia Grosicka-Maciag
Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
Iwonna Rahden-Staroń
Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
Jadwiga Turło
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland

ABSTRACT

Numerous formulations derived from the shiitake medicinal mushroom, Lentinus edodes, demonstrate anticancer activities. We hypothesized that isolates from selenium (Se)-enriched mycelia of L. edodes would possess stronger cancer-preventive properties than current preparations. The aim of this study was to investigate whether the presence of Se-methyl-seleno-L-cysteine in mycelial extracts of L. edodes affects their cytotoxic activity (makes them stronger) or whether they are as effective as Se-containing polysaccharides. Extracts were prepared from Se-containing mycelia under various conditions and assayed for cytotoxic activity in cancer (PC3 and HeLa) and normal (HMEC-1) cell lines. The chemical composition of the extracts was examined; specifically, the amounts of potentially cytotoxic Se compounds (methylselenocysteine, selenomethionine, and Se-containing polysaccharides) were measured. The relationship between extract composition and biological activity was characterized. Mycelial cultures were cultivated in a 10-L bioreactor in medium enriched with sodium selenite. Mycelial extracts were prepared either at 100°C or at 4°C in acidic solution. Total Se content was determined using the atomic absorption spectrometry method, and methylselenocysteine and selenomethionine contents were measured using reverse-phase high-performance liquid chromatography. Protein, carbohydrate, and polyphenolic contents were determined with spectrophotometric methods, and Se-containing polysaccharides were measured with the use of precipitation. Anticancer activity of mycelial extracts was examined using the MTT cell viability assay. Extracts containing Se-methyl-seleno-L-cysteine or Se-polysaccharides prepared at 4°C and 100°C, respectively, display moderate, time-dependent, specific cytotoxic activity in HeLa and PC3 cell lines. The effect in HeLa cells is more pronounced in the extract prepared at 4°C than at 100°C. The effect is almost equal for the PC3 cell line. However, both extracts have no effect or only slightly stimulate normal (HMEC-1) cell viability. The selective cytotoxic activity of L. edodes extracts in cancer (PC3 and HeLa) cells is due to the presence of both Se-methyl-seleno-L-cysteine and selenated polysaccharides, perhaps in combination with other active ingredients.


Articles with similar content:

Inhibitory Activity of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) on Transformed Cells by Human Papillomavirus
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 2
Vicente Madrid-Marina V., Elizur Montiel-Arcos, Ismael Leon-Rivera, Raul N. Ondarza-Vidaurreta, Enrique Garcia-Villa, Victor H. Bermudez-Morales, Eva Hernandez-Marquez, Patricio Gariglio, Ana I. Burgete-Garcfa, Alfredo Lagunas-Martinez
The Effect of Culture Liquid Ethyl Acetate Mycelium Extracts of Medicinal Mushrooms on the Viability of Human Pancreatic Cancer Cells
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 2
Solomon P. Wasser, Lital E. Sharvit, Fuad Fares
In Vitro Anti-proliferative Effects on NB4 Human Leukemia Cells and Physicochemical Screening of Pleurotus sp. (Higher Basidiomycetes) Mycelia from Cuba
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 3
Edgar Hernandez, Maria Cristina Tejedor, Ana Isabel Garcia-Perez, Pilar Sancho, Angel Herraez, Humberto J. Morris, Gabriel Llaurado, Jose Carlos Diez, Maria del Carmen Boyano-Adanez
Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Inhibits Candida Biofilms: A Metabolomic Approach
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 8
Kshipra Misra, Ajai Kumar, Jigni Mishra, Payal Gupta, Navin Kumar, Anuja Bhardwaj, Rakhee
Identification and Evaluation of Bioactivity of Compounds from the Mushroom Pleurotus nebrodensis (Agaricomycetes) against Breast Cancer
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 9
Xueya Zhang, Xiaohong Kou, Wancong Yu, Ru Wang, Zhaohui Xue, Jingfeng Hao