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International Journal of Medicinal Mushrooms

Impact factor: 1.211

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i3.90
pages 325-332

Antigenotoxic Potential of Aqueous Extracts from the Chanterelle Mushroom, Cantharellus cibarius (Higher Basidiomycetes), on Human Mononuclear Cell Cultures

Claudia Mendez-Espinoza
Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Centro Nacional de Investigacion Disciplinaria en Conservacion y Mejoramiento de Ecosistemas Forestales, Coyoacan, Mexico
Edelmira Garcia-Nieto
Centro de Investigacion en Genetica y Ambiente, Universidad Autonoma de Tlaxcala, Tlaxcala, Mexico
Adriana Montoya Esquivel
Centro de Investigacion en Ciencias Biologicas, Universidad Autonoma de Tlaxcala, Tlaxcala, Mexico
Monica Montiel Gonzalez
Centro de Investigacion en Ciencias Biologicas, Universidad Autonoma de Tlaxcala, Tlaxcala, Mexico
Efrain Velasco Bautista
Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Centro Nacional de Investigacion Disciplinaria en Conservacion y Mejoramiento de Ecosistemas Forestales, Coyoacan, Mexico
Carmen Calderon Ezquerro
Universidad Nacional Autonoma de Mexico, Centro de Ciencias de la Atmosfera, Mexico City, Mexico
Libertad Juarez Santacruz
Centro de Investigacion en Genetica y Ambiente, Universidad Autonoma de Tlaxcala, Tlaxcala, Mexico

ABSTRACT

Cantharellus cibarius is one of the most important wild, edible, and ectomycorrhizal mushrooms growing at La Malinche National Park, Tlaxcala, Mexico; therefore, the assessment of its biological properties is of great interest to know its potential as an alternative treatment to chemopreventive strategies when it is consumed as part of a diet. Comet assay was used to evaluate the antigenotoxic properties of several concentrations of aqueous extracts (0.0125, 0.025, 0.05, 0.1, and 0.2% w/v) prepared at room temperature (22 ± 2°C). As a test system we used human mononuclear cells exposed to methyl methanesulphonate (MMS) in vitro according to 3 different protocols: previous, simultaneous, and posterior. Previous (0.0125%) and simultaneous (0.1%) treatments resulted in the highest inhibitory efficiency. In the former, the cells assessed showed a tail length of 94.9 ± 64 µm; in the latter, the tails measured 106.2 ± 40 µm. Resulting percentages of reduction in damage were 236% and 196.1%, respectively. We did not obtain a dose-dependent response. The mean tail length for each protocol (previous, 133.1 ± 80 µm; simultaneous, 127.8 ± 57 µm; posterior, 146.3 ± 74 µm) was statistically significant with regard to the positive control (MMS).