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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i4.60
pages 395-408

Antioxidant and Antigenotoxicity Activities of Extracts from Liquid Submerged Culture of Culinary-Medicinal Ferula Oyster Mushroom, Pleurotus eryngii (DC.) Quél. var. ferulae (Lanzi) Sacc. (Agaricomycetideae)

Shu-Hui Hu
Department of Nutrition and Dietetics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan
Juang-Lin Lien
Department of Seafood Science, National Kaohsiung Marine University, No. 142, Haijhuan Rd., Kaohsiung, Taiwan, 807
Shu-Ling Hsieh
Department of Nutrition and Health Science, Fooyin University, Ta-liao Shiang, Kaohsiung, Taiwan, 831
Jinn-Chyi Wang
Department of Food Science and Technology, Tajen University, Shin-Erh Village, Yen-Pu Shiang, Pingtung, Taiwan
Sue-Joan Chang
Department of Life Science, National Cheng Kung University, Tainan, Taiwan, 701

ABSTRACT

The ferula oyster mushroom (Pleurotus eryngii var. ferulae) is medicinal, edible, and delicious. Response surface methodology was used to determine the optimal conditions for the production of P. eryngii var. ferulae biomass in liquid submerged culture. Lyophilized mycelium was subjected to two water extracts and two elutes through column chromatography using methanol-ethyl acetate-dichloromethane (referred to as EAM and CHE, respectively). In addition, the crude protein and water-soluble polysaccharides were extracted from the cultured broth. Six samples were tested to explore their antigenotoxicity with the Ames test and a rec-assay, as well as antioxidant activities in in vivo and in vitro tests. EAM had the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity and ferric-reducing ability. Moreover, hamster serum showed the highest activities of glutathione peroxidase and superoxide dismutase after EAM was administered at a dose of 0.3 g kg−1 body weight daily among all tested samples. Meanwhile, the EAM group displayed significantly higher activities of the two enzymes than did the control group. In the antimutagenicity test, EAM exhibited the highest inhibition rate against every tested mutagen at a low concentration of 0.08 mg mL−1 and a high concentration of 0.3 mg mL−1 in each plate, and it also had the highest anti-DNA-damaging activity among all test samples. EAM displayed the highest antigenotoxicity effect, followed by CHE, when using water-insoluble extracts, and CP was the highest one, followed by water extract (100°C, 30 minutes), water extract (60°C, 30 minutes), and SPPF, when using water-soluble extracts. The major constituents of EAM and CHE were identified as ergosterol and 2-pentanol, respectively. Some extracts from P. eryngii var. ferulae have significant antioxidant activity and are strong antigenotoxic agents.


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