Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
Indexed in
β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages
ABSTRACT
Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction. The monosaccharide composition of the SK5 fraction revealed by gas chromatography−mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted. In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice. Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity. There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide. The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
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Toyoshima Takashi, Ishibashi Ken-ichi, Yamanaka Daisuke, Adachi Yoshiyuki, Ohno Naohito, Resistance of <I>Aspergillus fumigatus</I> to Micafungin is Increased by Exogenous β-glucan, Medical Mycology Journal, 58, 1, 2017. Crossref
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Fan Hong-Bo, Zou Yuan, Han Qing, Zheng Qian-Wang, Liu Ying-Li, Guo Li-Qiong, Lin Jun-Fang, Cordyceps militaris Immunomodulatory Protein Promotes the Phagocytic Ability of Macrophages through the TLR4-NF-κB Pathway, International Journal of Molecular Sciences, 22, 22, 2021. Crossref
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Gallo A. L., Soler F., Pellizas C., Vélez M. L., Polysaccharide extracts from mycelia of Ganoderma australe: effect on dendritic cell immunomodulation and antioxidant activity, Journal of Food Measurement and Characterization, 16, 4, 2022. Crossref