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International Journal of Medicinal Mushrooms
IF: 1.211 5-Year IF: 1.394 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v10.i1.50
pages 37-48

High Immunomodulatory and Preventive Effects Against Sarcoma 180 in Mice Fed with Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Mycelium

Rosalia Rubel
Bioprocess Engineering and Biotechnology Division, Federal University of Paraná, Curitiba, Paraná, Brazil
Herta Stutz Dalla Santa
Bioprocess Engineering and Biotechnology Division, Federal University of Paraná, Curitiba, Paraná, Brazil
Luiz Claudio Fernandes
Department of Physiology, Federal University of Paraná, Curitiba, Paraná, Brazil
Jose Hermenio Cavalcante Lima Filho
Department of Medical Pathology Federal, University of Parana (UFPR), Curitiba, Parana, Brasil
Bonald Cavalcante Figueiredo
Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil
Raffaello Di Bernardi
Department of Biological Sciences , University of Parana (UFPR), Curitiba, Parana, Brazil
Andrea N. Moreno
Biology Course, University Catholic of Parana, Curitiba, Parana, Brazil
Fan Leifa
Institute of Horticulture, Academy of Agronomy Sciences of Zhejiang, Hangzhou, Zhejiang Province, China
Carlos Ricardo Soccol
Bioprocess Engineering and Biotechnology Division, Federal University of Paraná, Curitiba, Paraná, Brazil

ABSTRACT

This study evaluated the immunomodulatory and antitumor effects of a diet supplemented with Ganoderma lucidum (Ling Zhi or Reishi) medicinal mushroom mycelium obtained by solid-state fermentation. Over 14 weeks, animals from a test group were fed with 2 concentrations of G. lucidum mycelium, at 85% or 50%, labeled G85 and G50 diets, respectively. A control group received a regular diet. In the tenth week, half the animals from the test group and half the animals from the control group were inoculated with a suspension of Sarcoma 180 cells. Saline was injected in the remaining animals. In week 14, plasma levels of cytokines and the population of CD3+, CD4+, CD8+, CD19+, and CD16+/ CD32+ cells were evaluated. When compared to the control group, G. lucidum-supplemented diets altered the immune systems of the mice significantly (p < 0.05) the G50 diet proving more effective than the G85 diet. Mice inoculated with saline and fed with the G50 diet presented an increase in CD19+ and CD16+/CD32+ cell populations, along with an elevation in the tumor necrosis factor (TNF)-α and interferon (IFN)-γ concentrations. Conversely, tumor-bearing mice fed with the G50 diet (labeled the G50-S group) presented an increase in CD3+, CD4+, and CD8+ cells, but a decrease in CD19+ and CD16+/ CD32+ cell populations. TNF-α and IFN-γ concentrations were reduced in the G50-S group. The G50-S group presented a tumor weight 84% lower than that found in the control group. These results suggest that the immunomodulatory effect provoked by the consumption of G. lucidum mycelium possibly increased mice resistance against Sarcoma 180.