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ISSN Print: 1521-9437
ISSN Online: 1940-4344
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Antitumor Properties of Submerged Cultivated Biomass and Extracts of Medicinal Mushrooms of Genus Hypsizygus Singer (Agaricomycetideae)
ABSTRACT
The present article describes the development of media for high-yield cultivation of medicinal mushrooms Hypsizygus marmoreus and H. ulmarius and the results of in vivo investigations into the antitumor activity of the water extracts of the mycelia of both species and of the total polysaccharide fraction of H. ulmarius. The media were optimized using statistical experimental design methodologies, including full factorial design and steepest ascent. The mycelia yield of H. ulmarius amounted to 32-33 g/L of air-dried biomass on the 3rd to 4th day of cultivation. The corresponding figures for two strains of H. marmoreus, Hm-1 and Hm-2, were 26 and 17 g/L, respectively, on the 7th day of cultivation. The water content of air-dried biomass did not exceed 5%. The antitumor activity was studied in vivo on adult male hybrid mice (C57Bl/6 × DBA/2)F1 with transplantable tumor T-cell lymphoma P388. The inhibition ratio (IR) of tumor growth reached 80% for the water extract of H. ulmarius mycelium and 38% and 59%, respectively, for the two strains of H. marmoreus. The water-soluble polysaccharide fraction of H. ulmarius mycelium contained glucose, galactose, mannose, rhamnose, arabinose, and xylose in the relative amounts of 23.5, 9.5, 7, 2, 1.5, and 1, respectively; its antitumor activity was tested in 2 doses, 2 and 20 mg/kg/day. The 2 mg/ kg/day dose was more effective than either the 20 mg/kg/day dose or the mycelium water extract. The results prove H. ulmarius to be a promising source of antitumor polysaccharides.
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Qiu Chengshu, Yan Wenjuan, Deng Wangqiu, Song Bin, Li Taihui, Genetic Diversity Analysis ofHypsizygus marmoreuswith Target Region Amplification Polymorphism, The Scientific World Journal, 2014, 2014. Crossref
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Wu Ying-Ying, Shang Jun-Jun, Li Yan, Zhou Chen-Li, Hou Di, Li Jia-Li, Tan Qi, Bao Da-Peng, Yang Rui-Heng, The complete mitochondrial genome of the Basidiomycete edible fungus Hypsizygus marmoreus, Mitochondrial DNA Part B, 3, 2, 2018. Crossref
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Öztürk Ceren, Atila Funda, Changes in lignocellulosic fractions of growing substrates during the cultivation of Hypsizygus ulmarius mushroom and its effects on mushroom productivity, Scientia Horticulturae, 288, 2021. Crossref
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Krasnopolskaya Larissa, Shuktueva Maria, Golyshkin Aleksandr, Almyasheva Nailya, Yarina Maria, Optimization of the Nutrient Medium for Flammulina velutipes Submerged Biomass Production and Micromorphology of Its Mycelium, Fermentation, 7, 3, 2021. Crossref
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