Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
Indexed in
Effects of Carbon and Nitrogen Sources in the Medium on Tremella mesenterica Retz.:Fr. (Heterobasidiomycetes) Growth and Polysaccharide Production
ABSTRACT
The effects of different carbon and nitrogen sources, as well as of the initial medium pH, on Tremella mesenterica growth and polysaccharide production in submerged cultivation was investigated. Maximal biomass and extracellular polysaccharide (EPS) production was achieved when using sucrose, maltose, or mannitol as the only carbon source and peptone or corn steep liquor as the nitrogen source (yield of crude mycelium-polysaccharide ethanol precipitate reached 21–23 g/L). It has been shown that the yield of biomass and polysaccharides correlates with carbon source concentration in the medium. The EPS production improved threefold with increasing initial sucrose concentration from 1 to 6%. The levels of EPS production by the fungus grown at an initial pH of 5.0, 6.0, and 7.0 were, respectively, 11.7, 11.0, and 6.7 g/L. The cultivation process in shake flasks could be successfully reproduced in a laboratory bioreactor. The maximum mycelium-polysaccharide precipitate yield reached in 3 and 4 days of fermentation was 28.3 and 31.4 g/L, respectively.
-
Wu Xiaojun Jeffrey, Hansen Conly, Effects of Whey Permeate-Based Medium on the Proximate Composition of Lentinus edodes in the Submerged Culture, Journal of Food Science, 71, 6, 2006. Crossref
-
Wu X.J., Hansen C., Antioxidant Capacity, Phenolic Content, and Polysaccharide Content of Lentinus edodes Grown in Whey Permeate-Based Submerged Culture, Journal of Food Science, 73, 1, 2007. Crossref
-
Fazenda Mariana L., Seviour Robert, McNeil Brian, Harvey Linda M., Submerged Culture Fermentation of “Higher Fungi”: The Macrofungi, in Advances in Applied Microbiology Volume 63, 63, 2008. Crossref
-
Wang Yaqi, Bao Li, Yang Xiaoli, Li Li, Li Shaifei, Gao Hao, Yao Xin-Sheng, Wen Huaan, Liu Hong-Wei, Bioactive sesquiterpenoids from the solid culture of the edible mushroom Flammulina velutipes growing on cooked rice, Food Chemistry, 132, 3, 2012. Crossref
-
Yap Kevin Yi-Lwern, Lim Ken Juin, Merging the old with the new: a cybermedicine marriage for oncology interactions with traditional herbal therapies and complementary medicines, TANG [HUMANITAS MEDICINE], 2, 2, 2012. Crossref
-
N. Anike Felicia, Isikhuemhen Omoanghe S., Blum Dietrich, Neda Hitoshi, Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>, Advances in Bioscience and Biotechnology, 06, 08, 2015. Crossref
-
Corrêa Rúbia Carvalho Gomes, da Silva Bruna Polacchini, Castoldi Rafael, Kato Camila Gabriel, de Sá-Nakanishi Anacharis Babeto, Peralta Rosely Aparecida, de Souza Cristina G. M., Bracht Adelar, Peralta Rosane Marina, Spent mushroom substrate of Pleurotus pulmonarius: a source of easily hydrolyzable lignocellulose, Folia Microbiologica, 61, 5, 2016. Crossref
-
Enshasy Hesham El, Immunomodulators, in Industrial Applications, 2011. Crossref
-
Choi Jang Won, Ra Kyung Soo, Kim Seong Yeong, Yoon Taek Joon, Yu Kwang-Won, Shin Kwang-Soon, Lee Sam Pin, Suh Hyung Joo, Enhancement of anti-complementary and radical scavenging activities in the submerged culture of Cordyceps sinensis by addition of citrus peel, Bioresource Technology, 101, 15, 2010. Crossref
-
El Enshasy Hesham, Agouillal Farid, Mat Zarani, Malek Roslinda Abd, Hanapi Siti Zulaiha, Leng Ong Mei, Dailin Daniel Joe, Sukmawati Dalia, Pleurotus ostreatus: A Biofactory for Lignin-Degrading Enzymes of Diverse Industrial Applications, in Recent Advancement in White Biotechnology Through Fungi, 2019. Crossref
-
Singh M. P., Agarwal Sonam, Kushwaha Ankita, Chaturvedi Vivek K., Application and Biodegradation of Lignocellulosic Biomass, in Mycodegradation of Lignocelluloses, 2019. Crossref
-
He Huihui, Li Yingying, Fang Mingyue, Li Tiantian, Liang Yunxiang, Mei Yuxia, Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus, Journal of Microbiology and Biotechnology, 31, 6, 2021. Crossref
-
Ribeiro Bárbara, Andrade Paula B., Baptista Paula, Barros Lillian, Ferreira Isabel C. F. R., Seabra Rosa M., Valentão Patrícia, Leucopaxillus giganteus Mycelium: Effect of Nitrogen Source on Organic Acids and Alkaloids, Journal of Agricultural and Food Chemistry, 56, 12, 2008. Crossref
-
Petre Alexandru, Ene Mihaela, Vamanu Emanuel, Submerged Cultivation of Inonotus obliquus Mycelium Using Statistical Design of Experiments and Mathematical Modeling to Increase Biomass Yield, Applied Sciences, 11, 9, 2021. Crossref
-
Mao Xian-Bing, Zhong Jian-Jiang, Significant effect of NH4+ on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris, Enzyme and Microbial Technology, 38, 3-4, 2006. Crossref
-
Veiga M. C., Piazzi A. C. F., Mazutti M. A., Fermentation Processes for Production of Fungal Biopolymers with Industrial Applications, in Fungal Biopolymers and Biocomposites, 2022. Crossref