Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018028980
pages 1107-1119

Cross-Flow Ultrafiltration Fractions of a Cold Aqueous Extract of the Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), Exhibit Apoptosis in Tumor Cells

Miriam Sari
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Katharina Toepler
Bio-Pharmaceutical Chemistry and Molecular Pharmacology, Technische Hochschule Koeln, Leverkusen, Germany
Anna Nickisch-Hartfiel
Department of Chemistry, Niederrhein University of Applied Sciences, Krefeld, Germany
Nicole Teusch
Bio-Pharmaceutical Chemistry and Molecular Pharmacology, Technische Hochschule Koeln, Leverkusen, Germany
Reinhard Hambitzer
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany

ABSTRACT

Lentinus edodes (shiitake) ranks among the most well-known medicinal mushrooms worldwide. Immune-modulating effects of shiitake extracts have been widely demonstrated in animals and humans. Apart from providing highly purified compounds such as lentinan, crude mushroom extracts also show antitumor activities. The direct cytotoxicity of crude preparations still requires investigation. The focus of our study lies in the molecular weight cutoff distribution of β-glucans and proteins in a cold aqueous extract from L. edodes. We applied cross-flow ultrafiltration to obtain 6 fractions with different molecular sizes. β-glucan and protein contents were quantified. We were able to show that only small amounts of β-glucans were extracted and that protein content decreases with fraction size. The cytotoxic potential of the cold aqueous preparation was demonstrated by analyzing inhibition of the growth of non-small cell lung cancer and triple-negative breast cancer cells.


Articles with similar content:

Characterization of Cross-Flow Ultrafiltration Fractions from Maitake Medicinal Mushroom, Grifoia frondosa (Agaricomycetes), Reveals Distinct Cytotoxicity in Tumor Cells
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 8
Miriam Sari, Nicole Teusch, Katharina Toepler, Jan I. Lelley, Anna Nickisch-Hartfiel, Reinhard Hambitzer
Anti β-Glucan Antibody in Cancer Patients (Preliminary Report)
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 1
Yoshiyuki Adachi, Osamu Mizukami, Noriko N. Miura, Naohito Ohno, Ken-Ichi Ishibashi, Masuro Motoi
Selective Cytotoxic Activity of Se-Methyl-Seleno-L-Cysteine– and Se-Polysaccharide–Containing Extracts from Shiitake Medicinal Mushroom, Lentinus edodes (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 8
Maciej Dawidowski, Iwonna Rahden-Staroń, Marzenna Klimaszewska, Jadwiga Turło, Agnieszka Szczepanska, Dagmara Kurpios-Piec, Emilia Orzechowska, Piotr Podsadni, Emilia Grosicka-Maciag, Sandra Górska
Anti-β-Glucan Antibody in Bovine Sera
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 4
Yoshiyuki Adachi, Noriko N. Miura, Tsunenori Iriki, Yu-ichiro Kurone, Naohito Ohno, Ken-Ichi Ishibashi, Chikaku Dogasaki, Masuro Motoi
Chemopreventive Properties of Mushrooms Against Breast Cancer and Prostate Cancer
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Y.-C. Yuan, K. Lui, J. Ye, G. Hur, D. Smith, S. Chen, S. Phung, S. Kwok, K. Karlsberg, S. Oh