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International Journal of Medicinal Mushrooms
CiteScore™: 1.38 IF: 1.211 5-Year IF: 1.394 SNIP: 0.661 SJR: 0.433

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v9.i34.130
pages 385-394

Mycelial Growth of Three Pleurotus (Jacq.: Fr.) P. Kumm. Species on Sugarcane Bagasse: Production of Hydrolytic and Oxidative Enzymes

Gerardo Mata
Unidad de Micologia, Institute de Ecologia A.C., Apartado Postal 63, Xalapa 91000, Veracruz, Mexico
Erika Gonzalez Cortes
Instituto de Ecologia, A. C., Xalapa, Veracruz, Mexico
Dulce Salmones
Instituto de Ecologia, A. C., Xalapa, Veracruz, Mexico

ABSTRACT

The present study is based on the mycelial growth of 37 Pleurotus strains, belonging to P. djamor, P. ostreatus, and P. pulmonarius species. The strains were grown on sugarcane bagasse, using 2 kinds of inoculation: (1) spawn prepared on wheat seeds, and (2) mycelium grown within solid water-agar culture with no nutrients added. Based on differences found in the mycelial growth, 6 strains were selected to determine the production of certain hydrolytic and oxidative enzymes throughout the full growth cycle at 0, 4, 8, 12, and 16 days (incubation period), also including the primordia formation, fruiting, and postharvest stages. The production of laccase and Mn-peroxidase was clear during the incubation stage, decreased during the fruiting stage, and increased again during the postharvest stage. In addition, the production of cellulases, cellobiohydrolases, and xylanases was low during the incubation period, increased during the production of fruiting bodies, and declined during the postharvest. There was a visible inverse correlation between the content of soluble phenols of the substrate and the laccase production at 8 (r = −.85) and 12 (r = −.92) incubation days. The incubation period varied between 12 and 45 days among the studied strains. Their biological efficiency was generally good, with values between 65.3% and 118.8%.