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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020034178
pages 359-366

Comparative Assessment of Antifungal and Antibacterial Activities of Crude Extracts of the King Tuber Culinary-Medicinal Mushroom, Pleurotus tuber-regium (Agaricomycetes) from Cameroon

Blondo-Pascal Metsebing
Mycological Laboratory, University of Yaoundé 1, B.P. 1456 Yaoundé, Cameroon; Department of Biotechnology and Food Technology, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa; Department of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa
Romuald Oba
Mycological Laboratory, University of Yaoundé 1, B.P. 1456 Yaoundé, Cameroon; Department of Biotechnology and Food Technology, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa; Department of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa
Dominique Claude Mossebo
Mycological Laboratory, University of Yaoundé 1, B.P. 1456 Yaoundé, Cameroon
Thierry Youmbi Fonkui
Department of Biotechnology and Food Technology, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa
Fabrice Tsigaing Tsigain
Mycological Laboratory, University of Yaoundé 1, B.P. 1456 Yaoundé, Cameroon
Marthe Carine Djuidje Fotsing
Department of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa
Tata Charlotte Mungoh
Department of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa
Derek Ntantoh Ndinteh
Department of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein Campus 2028, South Africa

ABSTRACT

Antifungal and antibacterial activities of crude extracts of carpophore compared with those of sclerotium of Pleurotus tuber-regium were investigated on 11 species of bacterial and 3 fungal human pathogens. The minimal inhibitory concentration (MIC) of carpophore extract was recorded to be 12.5 mg/mL on Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Escherichia cloacae, Proteus mirabilis, P. vulgaris, Klebsiella oxytoca, and K. aerogenes and 6.25 mg/mL on Staphylococcus aureus, Escherichia coli, and Mycobacterium smegmatis as well as on all three species of fungal pathogens including Candida albicans, Aspergillus fumigatus, and A. ochraceus. In comparison, the MIC of sclerotium was recorded to be 12.5 mg/mL on Bacillus subtilis and Klebsiella aerogenes; 6.25 mg/mL on Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Enterobacter cloacae, E. coli, Mycobacterium smegmatis, Proteus mirabilis, P. vulgaris, and Klebsiella oxytoca; and 3.13 mg/mL on the three fungal pathogens. Based on the abovementioned figures, it appears that strains of pathogenic fungi tested are much more sensitive to crude extracts than the abovementioned bacteria. In fact, antimicrobial activities of crude extracts of P. tuber-regium, no matter whether it is that of the carpophore or its sclerotium, are in general stronger on human pathogenic fungi than bacteria. These figures also demonstrate that crude extracts of sclerotium show a higher antimicrobial activity than that of carpophore. Carpophores and sclerotia of P. tuber-regium could therefore constitute a source of new molecules potentially more efficient than synthetic products against some human pathogenic fungi and bacteria.

REFERENCES

  1. Alves MJ, Ferreira IC, Dias J, Teixera V, Martins A, Pintado M. A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Plant Med. 2012;78:1707-18.

  2. Smania AJ, Delle Monache F, Smania EFA, Cueno RS. Antibacterial activity of steroidal compounds isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetidae) fruit body. Int J Med Mushrooms. 1999;1:325-30.

  3. Imtiaj A, Jayasinghe C, Woo Lee G, Soo Lee T. Antibacterial and antifungal activities of Stereum ostrae, an inedible wild mushroom. Mycobiology. 2007;35(4):1-10.

  4. Ofodile LN, Uma NU, Kokubun T, Grayer RJ, Ogundipe OT, Simmonds MS. Antimicrobial activity of some Ganoderma species. Phytother Res. 2005;19:310-13.

  5. Moradali MF, Mostafavi H, Hejaroude GA, Tehrani AS, Abbasi M, Ghods S. Investigation of potential antibacterial properties of methanol extracts from fungus Ganoderma applanatum. Chemother. 2006;52(5):241-44.

  6. Synytsya A, Monkai J, Bleha R, Macurkova A, Ruml T, Ahn J, Chukeatirote E. Antimicrobial activity of crude extracts prepared from fungal mycelia. Asian Pac J Trop Biomed. 2017;7:257-61.

  7. Badalyan SM, Isikhuemhen OS, Gharibyan NG. Antagonistic/antifungal activity of Pleurotus tuberregium (Fr.) Singer against selected fungal pathogens. Int J Med Mushrooms. 2008;10(2):155-62.

  8. Adebayo EA, Martinez-Carrera D, Morales P. Comparative study of antioxidant and antibacterial properties of the edible mushrooms Pleurotus levis, P. ostreatus, P. pulmonarius and P. tuber-regium. Int J Food Sci Technol. 2018;53(5):1316-30.

  9. Subrata G, Gunjan B, Prakash P, Subhash CM, Krishnendu A. Antimicrobial activities of basidiocarps of wild edible mushrooms of west Bengal, India. Int J Pharm Tech Res. 2012;4(4):1554-60.

  10. Hassan IAF, Zubaida KC, Khan I, Saleh AA. Comparative study of antibacterial activity of wood-decay fungi and antibiotics. J Bangladesh Pharm Soc. 2011;6:14-17.

  11. Biswas G, Rana S, Acharya K. Cardioprotective activity of ethanolic extract of Astraeus hygrometricus (Pers.) Morg. Pharmacologyonline. 2011;2:808-17.

  12. Poucheret P, Fons F, Rapior S. Biological and pharmacological activity of higher Fungi: 20-year retrospective analysis. Cryptogam Mycol. 2006;27(4):311-33.

  13. Badalyan SM, Barkhudaryan A, Rapior S. Recent progress in research on the pharmaceutical potential of mushrooms and prospects of their clinical application. In: Agrawal DC, Dhanasekaran M, editors. Medicinal mushrooms, recent progress in research and development. 1st ed. Springer Nature: Singapore; 2019. p. 1-71.

  14. Donatini B. Les vertus medicinales des champignons, Imprimerie Gamma a Trinqueux (51), M.I.F. s.a., Cormontreuil, France, 1999.

  15. Biswas G, Sarkar S, Acharya K. Hepatoprotective activity of the ethanolic extract of Astraeus hygrometricus (Pers.) Morg. Digest J Nanomater Biostruct. 2011;6(2):637-41.

  16. Okolo KO, Orisakwe OE, Siminialayi IM. Nephroprotective and antioxidant effects of king tuber oyster medicinal mushroom, Pleurotus tuberregium (Agaricomycetes), on carbon tetrachloride-induced nephrotoxicity in male Sprague Dawley rats. Int J Med Mushrooms. 2018;20(5):419-29.

  17. Valverde ME, Hernandez-Perez T, Paredes-Lopez O. Edible mushrooms: Improving human health and promoting quality life. Int J Microbiol. 2015;37:63-87.

  18. Iwalokun BA, Otunba AA, Olukoya DK. Comparative phytotechnical evaluation, antimicrobial and antioxidant properties of Pleurotus ostreatus. Afr J Biotechnol. 2007;5:1732-39.

  19. Tonjock RK, Mih A. Secondary metabolites of oil palm isolates of Ganoderma zonatum Murill. from Cameroon and their cytotoxicity against five human tumour cell lines. Afr J Biotechnol. 2011;10(42):8440-47.

  20. Waithaka PN, Gathuru EM, Githaiga BM, Onkoba KM. Antimicrobial activity of mushroom (Agaricus bisporus) and fungal (Trametes gibbosa) extracts from mushrooms and fungi of Egerton Main Campus, Njoro Kenya. J Biomed Sci. 2017;6(3):1-6.

  21. Chelela BL, Chacha M, Matemu A. Antibacterial and antifungal activities of selected wild mushrooms from Southern Highlands of Tanzania. Am J Res Commun. 2014;2(9):58-68.

  22. Oyetayo OV. Medicinal uses of mushrooms in Nigeria: Towards full and sustainable exploitation. Afr J Trad Complement Alternat Med. 2011;8(3):267-74.

  23. Guissou KLM, Sanon E, Sankara Ph, Guinko S. La mycotherapie au Burkina Faso: Etat des lieux et perspectives. J Appl Biosci. 2014;79:6896-6908.

  24. Walleyn R, Rammeloo J. The poisonous and useful fungi of Africa south of Sahara: A literature survey. National Botanic Garden of Belgium. 1994;10:1-56.

  25. Ijeh II, Okwujiako IA, Nwosu PC, Nnodim HI. Phytochemical composition of Pleurotus tuber-regium and effect of its dietary incorporation on body/organ weights and serum triacylglycerols in albino mice. J Med Plant Res. 2009;3(11):939-43.

  26. Boonsong S, Klaypradit W. Antioxidant activities of extracts from five edible mushrooms using different extractants. Agric Nat Resour. 2016;50:89-97.

  27. McFarland. The nephelometer: An instrument for estimating the numbers of bacteria in suspensions used for calculating the opsonic index for vaccines. JAMA. 1907;49:1176-78.

  28. Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;64:711-13.

  29. Maragesi SM, Nyamwisenda NT, Mwangomo D, Kidukuli A. In vitro antimicrobial activity and determination of essential metal and ash value contents of Trichodesma zeylanicum. Int J Res Pharmacol Pharmacother. 2013;2:417-24.

  30. Ishaku LE, Botha FS, McGway LJ, Eloff JN. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extract. BMC Complement Alternat Med. 2017;17(133):1-10.

  31. Praptiwi, Raunsai M, Wulansari D, Fathoni A, Augusta A. Antibacterial and antioxidant activities of endophytic fungi extracts of medicinal plants from Central Sulawesi. J Appl Pharm Sci. 2018;8(8):69-74.

  32. Fonkui YT, Ikhile IM, Munganza MF, Fotsing DCM, Arderne C, Noundou SX, Krause MWR, Ndinteh TD, Njobeh BP. Synthesis, characterization and biological application of novel schiff bases of 2-(trifluoromethoxy) alanine. J Chin Pharm Sci. 2018;27(5):307-23.

  33. Algiannis N, Kalpotzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of essential oils of two Origanum species. J Agric Food Chem. 2001;40:4168-70.


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