Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom <i>Ganoderma lucidum</i> (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)

George G. Songulashvili; Vladimir Elisashvili; Eviatar Nevo; Yitzhak Hadar

doi:10.1615/IntJMedMushr.v10.i4.90

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Home > Journals > International Journal of Medicinal Mushrooms > Volume 10, 2008 Issue 4 > Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)

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ISSN Print: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v10.i4.90
pages 361-368

Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)

George G. Songulashvili
Institute of Evolution, Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel; and Durmishidze Institute of Biochemistry and Biotechnology,0159 Tbilisi, Georgia

Vladimir Elisashvili
S. Durmishidze Institute of Biochemistry and Biotechnology of N.L.E. Georgian Agrarian University, Tbilisi 0159, Georgia

Eviatar Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel

Yitzhak Hadar
Department of Plant Pathology and Microbiology, Faculty of Agricultural and Environmental Quality Sciences, the Hebrew University, Rehovot 76100, Israel

ABSTRACT

The Ganoderma lucidum strain 447 was cultivated in a 10-L fermentor. We used the ethanol-production residue (by wheat) (REP) as the growth substrate and Cu as an inductor. Ganoderma lucidum was grown for 8 days before laccase reached the highest yield (188,600 U L⁻¹). The purification enzyme appeared as two-laccase isozyme bands on SDS-PAGE. The molecular masses were 43 and 56 kDa by SDS-PAGE. The pH optimum for 2,2'-azino-bis-[3-ethyltiazoline-6-sulfonate] (ABTS) oxidation was 3 in citric/acetic buffers. In this study, the optimum temperature for laccase activity was determined to be 30°C. The kinetic of laccase was experimented on using 12 phenolic substrates. The lowest Km values (0.0048 and 0.005 mM) were found for syringaldazine and ABTS, respectively.