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International Journal of Medicinal Mushrooms

Impact factor: 1.357

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i1.30
pages 19-25

The Shaggy Inc Cap Medicinal Mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) Substances Interfere with H2O2 Induction of the NF-κB Pathway through Inhibition of IκBα Phosphorylation in MCF7 Breast Cancer Cells

Mikheil D. Asatiani
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences & Institute of Evolution, University of Haifa; Department of Anatomy and Cell Biology, Faculty of Medicine, Technion, Haifa; Agricultural University of Georgia, Tbilisi
Solomon P. Wasser
Department of Evolutionary and Environmental Biology, Faculty of Natural Science, University of Haifa, Mount Carmel, Haifa, Israel; Institute of Evolution, University of Haifa, Mount Carmel, Haifa, Israel; N.G. Kholodny Institute of Botany, NAS of Ukraine, 2, Tereshchenkovskaya St., Kiev 01004, Ukraine
Eviatar Nevo
Institute of Evolution, and Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel
Nili Ruimi
Migal-Galilee Technology Center, Cancer Drug Discovery Program, Kiryat Shmona
Jamal A. Mahajna
Migal, Galilee Technology Center, Cancer Drug Discovery Program, Kiryat Shmona; Galilee Institute for Applied Research, Nazareth
Abraham Reznick
Department of Anatomy and Cell Biology, Faculty of Medicine, Technion, Haifa

ABSTRACT

Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the IC50 value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H2O2-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.