Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020036163
Forthcoming Article

Dichloromethane Extract of fermentation broth by co-culture of Morchella esculenta and Coprinus comatus induces apoptosis in U251 Cells via mitochondrial intrinsic pathway

Xue Zhong
Dalian University
Qian Li
Tsinghua university
Huiguo Wang
Dalian university
Xiaohong Zhao
Dalian university
Hongzhu Yu
Tsinghua university
Hongkun Xue
Tsinghua university
Xu Cai
Tsinghua university
Hua Wang
Tsinghua university
Xianwei Zou
Tsinghua university
Xuan Liu
Dezhou University
Benke Chen
Tsinghua university
Liya Li
China-Japan Friendship Hospital
Linyu Jia
Dalian university
Jintian Tang
Tsinghua university

ABSTRACT

The current study was designed to investigate the inhibitory effects of dichloromethane extract of fermentation broth by co-culture of Morchella esculenta and Coprinus comatus (DCMM) on human glioma U251 cells in vitro and its possible underlying mechanisms. The proliferation of U251 cells was inhibited by DCMM with different concentrations by the CCK-8 assay. Besides, flow cytometry assay was used to evaluate the DCMM promoted U251 cell apoptosis rate with a dose-dependent manner. DCMM with different concentrations (10 μg·mL-1, 20 μg·mL-1, and 40 μg·mL-1) significantly enhanced the expression of caspases 3 activity on 24 h. In addition, DCMM with different concentrations significantly increased caspase-3 and Bax, decreased Bcl-2 expressions at both mRNA and protein levels. The DCMM can remarkably inhibit the proliferation and promote cell apoptosis of human glioma U251 cells. The possible underlying mechanisms could be related to induce apoptosis of human glioma U251 cells by mitochondrial intrinsic pathway.