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International Journal of Medicinal Mushrooms
Dichloromethane Extract of fermentation broth by co-culture of Morchella esculenta and Coprinus comatus induces apoptosis in U251 Cells via mitochondrial intrinsic pathway
China-Japan Friendship Hospital
The current study was designed to investigate the inhibitory effects of dichloromethane extract of fermentation broth by co-culture of Morchella esculenta and Coprinus comatus (DCMM) on human glioma U251 cells in vitro and its possible underlying mechanisms. The proliferation of U251 cells was inhibited by DCMM with different concentrations by the CCK-8 assay. Besides, flow cytometry assay was used to evaluate the DCMM promoted U251 cell apoptosis rate with a dose-dependent manner. DCMM with different concentrations (10 μg·mL-1, 20 μg·mL-1, and 40 μg·mL-1) significantly enhanced the expression of caspases 3 activity on 24 h. In addition, DCMM with different concentrations significantly increased caspase-3 and Bax, decreased Bcl-2 expressions at both mRNA and protein levels. The DCMM can remarkably inhibit the proliferation and promote cell apoptosis of human glioma U251 cells. The possible underlying mechanisms could be related to induce apoptosis of human glioma U251 cells by mitochondrial intrinsic pathway.
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