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Catalysis in Green Chemistry and Engineering

ISSN Print: 2572-9896
ISSN Online: 2572-990X

Catalysis in Green Chemistry and Engineering

DOI: 10.1615/CatalGreenChemEng.2019029612
pages 345-355

PREPARATION OF COMBI-MAGNETIC CROSS-LINKED ENZYME AGGREGATES OF CELLULASE AND LIPASE

Girish S. Nhivekar
Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, Maharashtra, 400019, India
Virendra K. Rathod
Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India

ABSTRACT

Combi-magnetic cross-linked enzyme aggregates (CLEAs) of cellulase and lipase were successfully prepared by co-precipitation of cellulase and lipase using 80% ammonium sulphate and later cross-linking the enzyme aggregates with 100 mM glutaraldehyde cross-linker on amino-functionalized magnetic nanoparticles in 6 h. The synthesized nanoparticles and immobilized enzymes were analyzed and confirmed through X-ray diffraction and Fourier transform infrared. Various parameters affecting the immobilization such as precipitating agent, precipitant concentration, amount of cross-linker, and time of cross-linking were studied. The combi-magnetic CLEAs showed better stability at higher temperature and pH as compared to free enzymes. After immobilization, the reusability retained more than 90% for the first three cycles, which further reduced due to leaching. Vmax and Km values show that the immobilized enzyme was more active compared to the free form, while affinity of the enzyme was reduced.

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