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Visualization, Image Processing and Computation in Biomedicine


ISSN Online: 2162-3511

Archives: Volume 1, 2012 to Volume 2, 2013

Visualization, Image Processing and Computation in Biomedicine

DOI: 10.1615/VisualizImageProcComputatBiomed.2012004984

Label-free optical detection of optogenetic activation of cells using phase-sensitive Fourier domain optical coherence tomography

Niloy Choudhury
Biophotonics Laboratory, Department of Biomedical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931-1295, USA
Zhaoqiang Zhang
Stem Cells and Engineered Tissue Laboratory, Department of Biomedical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931-1295, USA
Feng Zhao
Stem Cells and Engineered Tissue Laboratory, Department of Biomedical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931-1295, USA
Ling Gu
Biophysics and Physiology Laboratory, Department of Physics, University of Texas at Arlington, 500 UTA Blvd., Arlington, Texas 76019, USA
Samar Mohanty
Biophysics and Physiology Group, Department of Physics University of Texas at Arlington, 500 UTA Blvd., Arlington, Texas 76019, USA
www.uta.edu/physics/

ABSTRACT

The existing methods for detection of neural activity during optogenetic stimulation are either mechanically intrusive or chemically invasive. Here, we report development of a label-free non-invasive optical readout method to monitor the changes in the cells resulting from optogenetic activation. Phase sensitive Fourier domain optical coherence tomography (PSFD-OCT) was deployed to detect the activation of Channelrhodpsin-2 sensitized cells under the stimulation of blue light. A monolayer of transfected human embryonic kidney cells (HEK 293) in a cell culture dish was activated using a fiber-coupled laser diode that was modulated by a square wave (frequency 30Hz with 50% duty cycle). Using the PSFD-OCT system, we demonstrate measurement of the optical path length change in the cell monolayer because of light-induced activation.