RT Journal Article
ID 055cd6b030698b7b
A1 Lian, Tiantian
A1 Dong, Caihong
A1 Yang, Tao
A1 Sun, Junde
T1 Three Types of Geranylgeranyl Diphosphate Synthases from the Medicinal Caterpillar Fungus, Cordyceps militaris (Ascomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2014
FD 2014-04-22
VO 16
IS 2
SP 115
OP 124
K1 medicinal mushrooms and fungi
K1 GGPPS
K1 Cordyceps militaris
K1 carotenoid
K1 prenyltransferases
K1 metabolism
AB Geranylgeranyl diphosphate synthase (GGPPS) is a key enzyme in the carotenoid biosynthetic pathway, catalyzing the synthesis of its C20 precursor. In the present study, three types of ggpps genes were cloned and analyzed from the Caterpillar Medicinal Fungus Cordyceps militaris, a valued carotenoid-producing species. The sequences were named as ggpps727, ggpps191, and ggpps595. The open reading frame codes for predicted polypeptides of 464, 550, and 431 aa. Three predicted GGPPSs had a high similarity to that from Beauveria bassiana ARSEF 2860 with identity of 73%, 71%, and 56%, respectively. Homology comparison of the deduced peptide sequences of the various GGPPSs revealed highly conserved domains. Both GGPPS727 and GGPPS191 from C. militaris contained all five domains highly conserved among prenyltransferases as well as two aspartate-rich DDXX(XX)D motifs in domains II and V, which have been proven essential for prenyltransferase activity. By constructing the phylogenetic tree of fungal GGPPSs, it was found that fungi-derived GGPPSs could be divided into three clusters, suggesting there were three types of GGPPSs in fungi. Each type may be responsible for a different metabolism. Three types of GGPPSs from C. militaris belonged to the different clusters separately. Expression analysis of three ggpps genes during the fruit body cultivation of C. militaris by real-time polymerase chain reaction (PCR) suggested the ggpps 191 gene may be involved in the synthesis of carotenoids and ggpps 727 may be responsible for primary metabolism. This is the first report of the GGPPS from C. militaris, a valued edible and medicinal fungus.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,3fd456332214676c,055cd6b030698b7b.html