RT Journal Article ID 1233d58a6d68bad2 A1 Rouf, Razina A1 Tiralongo, Evelin A1 Krahl, Anja A1 Maes, Karen A1 Spaan, Lina A1 Wolf, Stefan A1 May, Tom W. A1 Tiralongo, Joe T1 Comparative Study of Hemagglutination and Lectin Activity in Australian Medicinal Mushrooms (Higher Basidiomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2011 FD 2011-12-15 VO 13 IS 6 SP 493 OP 504 K1 medicinal mushrooms K1 Australian mushrooms K1 higher Basidiomycetes K1 fruit-bodies K1 hemagglutination K1 lectin K1 sugar specificity AB Fifteen Australian mushroom species (higher Basidiomycetes) were assessed for hemagglutination and lectin activity. Hemagglutination activity was evaluated using both neuraminidase treated and untreated rabbit and human A, B, and O erythrocytes. Lectin activity was determined by the ability of various mono- and oligosaccharides to inhibit hemagglutination activity. Of the mushrooms evaluated, seven contained lectin activity. However, five (Agaricus bitorquis, Chlorophyllum brunneum, Coprinus comatus, Cortinarius sp. TWM 1710, and Omphalotus nidiformis) expressed lectin activity in only one of two collections tested. The two remaining lectin active mushroom species (Phlebopus marginatus and Psathyrella asperospora) possessed lectin activity with the same sugar specificity in both collections. Although lectins were identified with diverse specificity, lactose-specific lectin activity was most frequently identified, being present in Agaricus bitorquis, Copronus comatus, Omphalotus nidiformis, and Phlebopus marginatus. In contrast, Psathyrella asperospora, Cortinarius sp. TWM 1710, and Chlorophyllum brunneum were found to possess lectin activity specific for N-acetyl-D-glucosamine, galactose, and N-acetyl-neurammic acid, respectively. Significantly, the galactose-specific lectin activity identified in Cortinarius sp. TWM 1710 and the lactose-specific lectin activity in Phlebopus marginatus have not been previously reported. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b5e519241ca8193,1233d58a6d68bad2.html