RT Journal Article
ID 1909db2128a9a661
A1 Zhang, Wenjuan
A1 Zhang, Xiaolong
A1 Li, Minghua
A1 Shi, Yan
A1 Zhang, Ping
A1 Cheng, Xian-Long
A1 Wei, Feng
A1 Ma, Shuangcheng
T1 Identification of Chinese Caterpillar Medicinal Mushroom, Ophiocordyceps sinensis (Ascomycetes) from Counterfeit Species
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2018-02-05
VO 19
IS 12
SP 1061
OP 1070
K1 internal transcribed spacer
K1 medicinal mushrooms and fungi
K1 Ophiocordyceps sinensis
K1 PCR-RFLP
K1 quality control
AB Ophiocordyceps sinensis is a valuable traditional Chinese medicine with a high market price. In this study,
a polymerase chain reaction–restriction fragment-length polymorphism (PCR-RFLP) method based on 2 enzymes was
developed to distinguish O. sinensis from 6 common counterfeit species. To verify the applicability of this method, we experimentally tested O. sinensis organisms, tablet preparations made from O. sinensis, and cultured mycelia isolated from O. sinensis. To validate the results from this PCR-RFLP method, all real samples were identified by internal transcribed spacer sequencing. This is, to our knowledge, the first time the PCR-RFLP method has been applied to identify O. sinensis. The selection of 2 restrictive enzymes for identification dramatically improved the accuracy and efficiency of this method. It is the great advantage of this method that sampling from either of 2 parts of O. sinensis—the fruiting body or the caterpillar body—would not cause any difference in the final experimental results. Therefore, this method is not only feasible for testing crude drugs of O. sinensis but it is also useful when the crude drugs are broken down into
powder or made into tablets, demonstrating the promising prospect of application in quality control.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2dab30ba797125f4,1909db2128a9a661.html