RT Journal Article
ID 24cfeb5406ee47ce
A1 Sari, Miriam
A1 Toepler, Katharina 
A1 Nickisch-Hartfiel, Anna 
A1 Teusch, Nicole 
A1 Hambitzer, Reinhard 
T1 Cross-Flow Ultrafiltration Fractions of a Cold Aqueous Extract of the Shiitake Culinary-Medicinal Mushroom, <i>Lentinus edodes</i> (Agaricomycetes), Exhibit Apoptosis in Tumor Cells
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-02
VO 20
IS 11
SP 1107
OP 1119
K1 B-glucans
K1 A549
K1 breast cancer
K1 cold water extraction
K1 cytotoxicity
K1 extract
K1 <i>Lentinus edodes</i>
K1 MDA-MB-231
K1 medicinal mushrooms
K1 non-small cell lung cancer
K1 ultrafiltration
K1 tumor cell growth
AB <i>Lentinus edodes</i> (shiitake) ranks among the most well-known medicinal mushrooms worldwide. Immune-modulating effects of shiitake extracts have been widely demonstrated in animals and humans. Apart from providing highly purified compounds such as lentinan, crude mushroom extracts also show antitumor activities. The direct cytotoxicity of crude preparations still requires investigation. The focus of our study lies in the molecular weight cutoff distribution of &beta;-glucans and proteins in a cold aqueous extract from <i>L. edodes</i>. We applied cross-flow ultrafiltration to obtain 6 fractions with different molecular sizes. &beta;-glucan and protein contents were quantified. We were able to show that only small amounts of &beta;-glucans were extracted and that protein content decreases with fraction size. The cytotoxic potential of the cold aqueous preparation was demonstrated by analyzing inhibition of the growth of non-small cell lung cancer and triple-negative breast cancer cells.


PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6e2de2a705b6ffd3,24cfeb5406ee47ce.html