%0 Journal Article %A Abidin, Mohamad Hamdi Zainal %A Abdullah, Noorlidah %A Abidin, Nurhayati Zainal %D 2016 %I Begell House %K medicinal mushrooms, oxidative stress, antioxidant, functional food, LDL oxidation, human aortic endothelial membrane damage %N 2 %P 109-121 %R 10.1615/IntJMedMushrooms.v18.i2.20 %T Protective Effect of Antioxidant Extracts from Grey Oyster Mushroom, Pleurotus pulmonarius (Agaricomycetes), Against Human Low-Density Lipoprotein Oxidation and Aortic Endothelial Cell Damage %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,3b344c674f08ce4b,313602623bcb1696.html %V 18 %X This study evaluated the in vitro antioxidant capacities of extracts from Pleurotus pulmonarius via Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, metal chelating, cupric ion reducing antioxidant capacity, and lipid peroxidation inhibition assays. Extract compositions were determined by phenol−sulfuric acid; Coomassie Plus (Bradford) protein; Spectroquant zinc, copper, and manganese test assays; and liquid chromatography−tandem mass spectrometry (LC/MS/MS) and gas chromatography−mass spectrometry (GC/MS). Methanol-dichloromethane extract, water fraction, hot water, aqueous extract and hexane fraction exhibited the most potent extracts in the antioxidant activities. LC/MS/MS and GC/MS showed that the extracts contained ergothioneine, ergosterol, flavonoid, and phenolic compounds. The selected potent extracts were evaluated for their inhibitory effect against oxidation of human low-density lipoproteins and protective effects against hydrogen peroxide−induced cytotoxic injury in human aortic endothelial cells. The crude aqueous extract was deemed most potent for the prevention of human low-density lipoprotein oxidation and endothelial membrane damage. Ergothioneine might be the compound responsible for the activities, as supported by previous reports. Thus, P. pulmonarius may be a valuable antioxidant ingredient in functional foods or nutraceuticals. %8 2016-04-18