RT Journal Article ID 37806b8d4f935af1 A1 Rouhana-Toubi, Amal A1 Wasser, Solomon P. A1 Agbarya, Abed A1 Fares, Fuad T1 Inhibitory Effect of Ethyl Acetate Extract of the Shaggy Inc Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes) Fruit Bodies on Cell Growth of Human Ovarian Cancer JF International Journal of Medicinal Mushrooms JO IJM YR 2013 FD 2013-10-17 VO 15 IS 5 SP 457 OP 470 K1 medicinal mushrooms K1 cell viability K1 Coprinus comatus K1 Shaggi Inc Cap medicinal mushroom K1 ovarian cancer K1 chromatography AB In a previous study, we screened organic extracts of different mushroom mycelia and picked ethyl acetate extract of Coprinus comatus as one of the most active extracts against human ovarian cancer cells. In the current study, we extracted a dry powder of C. comatus fruit bodies using ethyl acetate and examined its effect on the viability of three cell lines originated from human ovarian cancer (ES-2, SKOV-3, and SW-626). This extract was active against all tested cell lines, in a dose-dependent manner (concentrations 50−200 µg/mL, P<0.01). In an attempt to segregate the active fraction, we subjected the extract to chromatography on a silica gel column. The effect of six different fractions and of the crude extract on the viability of ES-2 cells was examined after exposure time of 24 h. Fraction F (last eluted) was significantly more effective than crude extract in the reduction of cell viability (P<0.01). Fraction F was also significantly more active than crude extract in the reduction of viability of SKOV-3 cells. We next identified some of the compounds of fraction F (mainly fatty acids) by gas chromatography−mass spectrometry. In summary, ethyl acetate extract of C. comatus reduced viability of three lines of human ovarian cancer. Fractionation of this extract by a silica gel column enabled the selection of a fraction significantly more active than the original extract. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7310c9d720cd3def,37806b8d4f935af1.html