RT Journal Article ID 3afed9126601702e A1 Brezáni, Adam A1 Svobodova, Katerina A1 Jablonský, Ivan A1 Tlustoš, Pavel T1 Cultivation of Medicinal Mushrooms on Spruce Sawdust Fermented with a Liquid Digestate from Biogas Stations JF International Journal of Medicinal Mushrooms JO IJM YR 2019 FD 2019-03-19 VO 21 IS 3 SP 215 OP 223 K1 alternative growth substrates K1 fermented conifer sawdust K1 Ganoderma lucidum K1 medicinal mushrooms K1 mushroom cultivation K1 Pleurotus spp. AB The aim of this work was to prepare a softwood substrate on which to grow edible and medicinal mushrooms. Liquid digestate from a biogas station was successfully used in spruce sawdust fermentation. Pleurotus ostreatus, P. eryngii, and Ganoderma lucidum were grown on the obtained substrates and their mycelia grew at rates similar to rates of growth on control beech sawdust; values ranged from 4.1 to 5.54 mm/day. A 6-week fermentation period was determined to be sufficient for removing volatile extractives from sawdust (76% removal efficiency), which elevated content was shown to be most critical for fungal growth. Removal of 47% of resinous compounds and a decrease in the carbon-to-nitrogen ratio in the growth substrate were found during sawdust fermentation in the presence of the liquid digestate. Among ligninolytic enzymes, the growth substrates produced here favored laccase produced by tested fungi. It follows that utilizing wastes from biogas production to reuse softwood wastes could make an environmentally friendly and economically viable biotechnology for producing mushrooms. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,5920db303fdf6d4c,3afed9126601702e.html