RT Journal Article
ID 446bcba113087c10
A1 Pasquini, Rossana
A1 Scassellati-Sforzolini, Giuseppina
A1 Villarini, Milena
A1 Moretti, Massimo
A1 Marcarelli, Massimiliano
A1 Fatigoni, Cristina
A1 Kaur, Satwinderjeet
A1 Kumar, Subodh
A1 Grover, Iqbal S.
T1 In Vitro Protective Effects of <i>Terminalia arjuna Bark</i> Extracts Against the 4-Nitroquinoline-</i>N</i>-Oxide Genotoxicity
JF Journal of Environmental Pathology, Toxicology and Oncology
JO JEP(T)
YR 2002
FD 2002-03-01
VO 21
IS 1
OP 12
AB We determined the antimutagenic potential of chloroform, acetone, methanol, methanol+HCl, diethyl ether, and ethyl acetate extracts of <i>Terminalia arjuna</i> bark against the model mutagen 4-nitroquinoline-<i>N</i>-oxide (4-NQO) using the <i>Salmonella</i>/microsome, comet, and micronucleus (MN) tests. <i>Salmonella typhimurium</i> TA100 strain and human peripheral white blood cells were coincubated with various concentrations (from 5 to 500 <font face=&quot;Symbol&quot;>m</font>g) of the six extracts and 4-NQO (from 0.05 to 2 <font face=&quot;Symbol&quot;>m</font>g). We found that the 4-NQO mutagenicity was inhibited by more than 70% in the <i>Salmonella</i>/microsome test at the highest nontoxic extract dose of ethyl acetate (50 <font face=&quot;Symbol&quot;>m</font>g/plate), chloroform (100 (<font face=&quot;Symbol&quot;>m</font>g/plate), acetone, (100 (<font face=&quot;Symbol&quot;>m</font>g/plate), and methanol (500 (<font face=&quot;Symbol&quot;>m</font>g/plate). A less marked antimutagenicity activity (inhibition of about 40&#8212;45%) was observed for the acidic methanol and diethyl ether extracts. The comet assay showed that acetone extract (100 <font face=&quot;Symbol&quot;>m</font>g/mL) was more effective in reducing the DNA damage caused by 4-NQO (ca. 90%), whereas the chloroform, ethyl acetate, and diethyl ether extracts were cytotoxic. In the MN test, the decrease in 4-NQO clastogenicity was observed by testing the mutagen especially with chloroform and ethyl acetate extracts (inhibition about 40-45%). The acetone and methanol extracts showed a less marked activity (33% and 37%, respectively). The results of the present study suggest that <i>T. arjuna</i> bark contains some nonpolar as well as polar compounds with antimutagenic activity against 4-NQO. Several explanations can be suggested, but further investigations are necessary to definitely identify the active compounds.
PB Begell House
LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,18179bf737097662,446bcba113087c10.html