RT Journal Article ID 4c8450d712e4dd4c A1 Younis, Ahmed M. A1 Wu, Fang-Sheng A1 El Shikh, Hussien H. T1 Antimicrobial Activity of Extracts of the Oyster Culinary Medicinal Mushroom Pleurotus ostreatus (Higher Basidiomycetes) and Identification of a New Antimicrobial Compound JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-06-30 VO 17 IS 6 SP 579 OP 590 K1 medicinal mushrooms K1 antimicrobial activity K1 oyster mushroom K1 Pleurotus ostreatus K1 mushroom extracts K1 3-(2-aminophenylthio)-3-hydroxypropanoic acid AB Pleurotus ostreatus is an edible mushroom that also has high medicinal values. In this study, P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The freeze-dried fruiting body, broth from submerged culture, and mycelial biomass of P. ostreatus were extracted using alcohols and water as solvents. The extracts were then tested for their antimicrobial activity against the growth of fungi and bacteria. It was observed that the water extract from fruiting bodies had the strongest effect in inhibiting the growth of most fungi. The most sensitive test microfungi to the inhibition were Candida albicans, Cryptococcus humicola, and Trichosporon cutaneum, and the most sensitive test bacteria were Staphylococcus aureus followed by Escherichia coli. Water extracts from culture broth or mycelial biomass were moderately inhibitive to the growth of fungi and bacteria. The alcohol-based solvents from all samples had much less antimicrobial activity against most test microorganisms. An antimicrobial compound was purified from the water extracts of fruiting bodies with Sephadex G 100 column chromatography and characterized by infrared absorption spectrum (IR), nuclear magnetic resonance (NMR), and mass spectroscopic analysis. We have identified this compound to be 3-(2-aminopheny1thio)-3-hydroxypropanoic acid. This purified compound had a minimum inhibitory concentration of 30 µg/mL and 20 µg/mL against the growth of fungi and bacteria, respectively. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,52f67ca6221cc3fa,4c8450d712e4dd4c.html