RT Journal Article ID 55bc86f512a65ade A1 Lu, Shu-Fang A1 Tsai, Shu-Chi A1 Lin, Hsiao-Chen A1 Fu, Weiguang T1 Study on the Analytical Method for Water-soluble Crude Polysaccharides in Culinary-Medicinal Mushrooms JF International Journal of Medicinal Mushrooms JO IJM YR 2010 FD 2011-02-04 VO 12 IS 4 SP 331 OP 345 K1 culinary-medicinal mushrooms K1 polysaccharides K1 analytical methods K1 Ganoderma tsugae K1 Trametes versicolor K1 Agaricus brasiliensis AB Size exclusion high performance liquid chromatography (SE-HPLC) was used to analyze water extracts of edible medicinal mushrooms in this research. Fingerprint profiling of polysaccharides in edible medicinal mushrooms was utilized as the primary identification tool for qualitative analysis. Furthermore, this study successfully improves or corrects the overestimation problem in quantifying functional polysaccharide contents in phenol-sulfuric acid method. The comparison of 3 different pre-treatment methods including SE-HPLC fractionation, ultra-filtration (UF) fractionation and alcohol precipitation shows that SE-HPLC fractionation method not only can recover the loss of polysaccharides in UF or alcohol precipitation methods, but also is able to fully retain water-soluble crude polysaccharides with molecular weight > 10 kD, which are commonly regarded as functional components in medicinal mushrooms. Therefore, the combination of SE-HPLC fractionation and phenol-sulfuric acid quantification method is more accurate and suitable for determination of water-soluble crude polysaccharide in edible medicinal mushrooms. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,0ca529c41b1c961c,55bc86f512a65ade.html