RT Journal Article ID 63a02050291c1286 A1 Zhang, Chun-Jing A1 Qi, Xiaodan A1 Shi, Yan A1 Sun, Yan A1 Li, Shuyan A1 Gao, Xiulan A1 Yu, Haitao T1 Optimization of Cultivation Conditions of Fermented Shaggy Ink Cap Culinary-Medicinal Mushroom, Coprinus comatus (O.Mull.:Fr.) Pers. (Higher Basidiomycetes) Rich in Vanadium JF International Journal of Medicinal Mushrooms JO IJM YR 2012 FD 2012-03-09 VO 14 IS 2 SP 189 OP 195 K1 medicinal mushrooms K1 vanadium K1 Coprinus comatus K1 medium K1 biomass K1 mycelia AB The present paper is mainly aimed at optimization of cultivation conditions of fermented mushrooms of Coprinus comatus rich in vanadium (CCRV). Initial screening of effects of carbon source, temperature, pH, and inoculum size were done by using a one-factor-at-a-time method. The results obtained in that study showed that the optimal medium composition was 30 g glucose/Lin YEPG medium, initial pH 6.0, inoculum volume 10%, and incubation time 120 h. Then the medium was subjected to screening of the most significant parameters using the L9 orthogonal array to solve multivariable equations simultaneously. The results obtained in this study showed that the optimal medium composition was 0.4% V and 30 g glucose/Lin YEPG medium, initial pH 5.0, inoculum volume 15%, and incubation time 120 h. At this medium composition, the mycelial biomass and V content were 7.18 ± 0.24 g/L and 3786.0 ± 17 μg/g, respectively. The anti-diabetic potential of CCRV produced with the optimal level was tested in alloxan-induced diabetes. After the mice were administered (i.g.) with CCRV, the level of blood sugar in the CCRV group was very close to that of the control group. These findings suggested that CCRV produced with the optimal level is useful in the control of diabetes mellitus. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,28491335273756b2,63a02050291c1286.html