RT Journal Article ID 6bbf6f1a1684c615 A1 Mal'ucka, Lucia Ungvarska A1 Harvanova, Jarmila A1 Pavlik, Martin A1 Rajtar, Martin A1 Jarosciak, Lukas T1 Free-Radical Scavenging Activities of Cultured Mycelia of Paecilomyces hepiali (Ascomycetes) Extracts and Structural Characterization of Bioactive Components by Nuclear Magnetic Resonance Spectroscopy JF International Journal of Medicinal Mushrooms JO IJM YR 2016 FD 2016-12-01 VO 18 IS 10 SP 895 OP 903 K1 1- and 2-dimensional NMR spectroscopy K1 cultivation K1 free radical scavenging activity K1 DPPH radical K1 medicinal mushroom and fungi K1 Paecilomyces hepiali AB Current research is focused on testing the cultivation of Paecilomyces hepiali mycelia on various plant substrates and producing fungus or mycelial biomass with qualitatively interesting substances. P. hepiali mycelia was cultivated using solid-state fermentation of different substrates. Mycelial biomass was then analyzed, and antioxidant activity was evaluated using the DPPH radical scavenging method for different ethanolic extracts based on a millet substrate (extract 1) or a chickpea substrate (extract 2). Extract 1 corresponds to a half-maximal DPPH radical inhibitory concentration of 1.73 mg/mL; the inhibitory concentration of ethanol extract 2 was almost 4.5 times higher at 7.92 mg/mL. Extracts 1 and 2 were separated into fractions by column chromatography and the chemical structures were determined for the substances that formed the most effective fraction of sample 1. The chemical structures of all compounds in the most active fraction of sample 1 were analyzed by 1H, 13C, distortionless enhancement by polarization transfer, correlation spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectra. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,531a42433150433b,6bbf6f1a1684c615.html