RT Journal Article
ID 737fe5d665d99c68
A1 Curtiss III, Roy
A1 Xin, Wei
A1 Li, Yuhua
A1 Kong, Wei
A1 Wanda, Soo-Young
A1 Gunn, Bronwyn
A1 Wang, Shifeng
T1 New Technologies in Using Recombinant Attenuated <i>Salmonella</i> Vaccine Vectors
JF Critical Reviews&trade; in Immunology
JO CRI
YR 2010
FD 2010-04-02
VO 30
IS 3
SP 255
OP 270
K1 antigen synthesis
K1 biological containment
K1 delayed attenuation or attenuation
K1 delayed
K1 recombinant vaccines
K1 <i>Salmonella</i>
K1 vaccine
AB Recombinant attenuated <i>Salmonella</i> vaccines (RASVs) have been constructed to deliver antigens from other pathogens to induce immunity to those pathogens in vaccinated hosts. The attenuation means should ensure that the vaccine survives following vaccination to colonize lymphoid tissues without causing disease symptoms. This necessitates that attenuation and synthesis of recombinant gene encoded protective antigens do not diminish the ability of orally administered vaccines to survive stresses encountered in the gastrointestinal tract. We have eliminated these problems by using RASVs with regulated delayed expression of attenuation and regulated delayed synthesis of recombinant antigens. These changes result in RASVs that colonize effector lymphoid tissues efficiently to serve as "factories" to synthesize protective antigens that induce higher protective immune responses than achieved when using previously constructed RASVs. We have devised a biological containment system with regulated delayed lysis to preclude RASV persistence in vivo and survival if excreted. Attributes were added to reduce the mild diarrhea sometimes experienced with oral live RASVs and to ensure complete safety in newborns. These collective technologies have been used to develop a novel, low-cost, RASV-synthesizing, multiple-protective <i>Streptococcus pneumoniae</i> antigens that will be safe for newborns/infants and will induce protective immunity to diverse <i>S. pneumoniae</i> serotypes after oral immunization.
PB Begell House
LK https://www.dl.begellhouse.com/journals/2ff21abf44b19838,1629d2c3507c27c5,737fe5d665d99c68.html