RT Journal Article
ID 7569c5f864d4acbb
A1 Zou, Yu
A1 Hou, Xiyan
T1 Extraction Optimization, Composition Analysis, and Antioxidation Evaluation of Polysaccharides from White Jelly Mushroom, Tremella fuciformis (Tremellomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2018-02-05
VO 19
IS 12
SP 1113
OP 1121
K1 antioxidant activity
K1 composition
K1 fraction
K1 medicinal mushrooms
K1 polysaccharide
K1 sonication-assisted
extraction
K1 Tremella fuciformis
AB We investigated the sonication-assisted extraction (SAE) conditions of Tremella fuciformis polysaccharides.
Single-factor experiments showed that the optimal sonication intensity was 6 W/cm2; the other SAE conditions
were further optimized using response surface experimental design. A Box-Behnken experiment exhibited that the optimal
SAE conditions were temperature, 85°C; ratio of liquid to solid, 46 mL/g; and sonication time, 2 hours. The maximal
polysaccharide yield (8.95 g/100 g) was obtained under the optimal extraction conditions. Polysaccharides extracted by
sonication or traditional hot water extraction (TWE) were further separated and purified using DEAE-Sepharose and
Sephadex G-100 column chromatography. Polysaccharide fractions (SAE-1, SAE-2, TWE-1, and TWE-2) were obtained;
their molecular weights were 9.85 × 104, 6.64 × 104, 4.75 × 105, and 2.81 × 105 Da, respectively. The total sugar content of SAE-1 and SAE-2 was lower and the protein and nucleic acid contents were higher than those of TWE-1 and TWE-2. In addition, SAE-1 and SAE-2 showed stronger Fe2+-chelating activities and superoxide radical scavenging activities than TWE-1 and TWE-2. This suggests that polysaccharides extracted by sonication possess high antioxidant activities.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2dab30ba797125f4,7569c5f864d4acbb.html