ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
Journal of Environmental Pathology, Toxicology and Oncology
インパクトファクター: 1.241 5年インパクトファクター: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2013007280
pages 189-203

Biochanin A Enhances the Radiotoxicity in Colon Tumor Cells In Vitro

Abhay Puthli
Department of Life Sciences, University of Mumbai, Mumbai, India; Radiological Physics & Advisory Division, Bhabha Atomic Research Centre, Mumbai, India
Reeta Tiwari
Department of Life Sciences, University of Mumbai, Mumbai, India; Radiological Physics & Advisory Division, Bhabha Atomic Research Centre, Mumbai, India
Kaushala Prasad Mishra
Department of Life Sciences, University of Mumbai, Mumbai, India; Nehru Gram Bharati University, Allahabad, UP, India; Foundation for Education and Research, India and BM International Research Centre, Mumbai, India

要約

Herbs and other plant-based compounds have increasingly been recognized as useful for the prevention and treatment of cancer. There exists enormous scope for screening and evaluation of herbal/plant products to develop an effective radiosensitizer and radioprotector that is relevant for cancer therapy. Anticancer agents that can effectively trigger the process of cell death in tumor cells need to be developed. This study describes the effect of the flavonoid biochanin A (BCA), administered alone or in combination with gamma radiation, on the growth of radioresistant human colon cancer HT29 cells in vitro. Proliferation studies were carried out using MTT assay with increasing concentration of BCA (1−100 µM) followed by gamma irradiation at a dose of 2 Gy. Induced reactive oxygen species, mitochondrial membrane potential, lipid peroxidation, and caspase-3 activation were measured by fluorescence assays and the magnitude of induced apoptosis in cells was evaluated by flow cytometry. Cellular DNA damage was determined by comet assay. Combined treatment caused a significant decrease in cell proliferation, a substantial increase in the generation of reactive oxygen species, enhanced lipid peroxidation, and increased mitochondrial membrane potential in treated HT29 cells compared with controls. Significantly enhanced apoptosis and DNA damage were found with a combination of drug and radiation treatments. Furthermore, it was found that combined treatment yielded an additive increase of caspase-3 in these cells. Our findings indicate that BCA acts as a remarkable pro-oxidant, significantly enhancing the radiotoxicity of colon cancer cells in vitro.


Articles with similar content:

Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 4
Di Wu, Wenbo Sun, Yanfang Liu, Yaping Zhang, Yan Yang, Henan Zhang, Li Min, Hairui Yang, Zheng Qiankun, Wei Jia, Wenhan Wang, Jinsong Zhang
Apoptotic Effect of Extract from Medicinal Mushroom from Taiwan Taiwanofungus salmoneus (Higher Basidiomycetes) Mycelium Combined with or without Cisplatin on Hepatocellular Carcinoma Cells
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Yun-Jung Hsieh, Jeng-Leun Mau, Rao-Chi Chien
Modification of Thymocytes Membrane Radiooxidative Damage and Apoptosis by Eugenol
Journal of Environmental Pathology, Toxicology and Oncology, Vol.23, 2004, issue 2
Kaushala Prasad Mishra, Badri Narain Pandey
Black Tea Protects Thymocytes in Tumor-Bearing Animals by Differential Regulation of Intracellular ROS in Tumor Cells and Thymocytes
Journal of Environmental Pathology, Toxicology and Oncology, Vol.24, 2005, issue 2
Sreya Chattopadhyay, Tanya Das, Arindam Bhattacharyya, Maqsood Siddiqi, Gaurisankar Sa, Lakshmishri Lahiry, Deba Prasad Mandal
Lutein Inhibits Cell Growth and Activates Apoptosis via the PI3K/AKT/mTOR Signaling Pathway in A549 Human Non-Small-Cell Lung Cancer Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.37, 2018, issue 4
Wen-long Zhang, Yuan-Song Bai, Zhang-zhen Shi, Dan Cong, Ya-nan Zhao