年間 4 号発行
ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537
Indexed in
The Influence of Nonprotein Thiols on DNA Damage Induced by Bleomycin in Single Human Cells
要約
Nonprotein thiols are considered radioprotectors, preventing DNA damage by ionizing radiation. Because bleomycin (BLM) is a radiomimetic agent, it was proposed that thiols may prevent DNA damage produced by this antibiotic. However, results obtained with treatments combining thiols and BLM in living cells are contradictory. The goal of this study was to analyze the DNA damage induced by BLM and the influence of 3 nonprotein thiols of different electrical charges and chemical compositions at the level of single cells (comet assay). We also studied the morphological signs of apoptosis produced by BLM in these same conditions. We found that all thiols potentiated DNA damage induced by BLM, most probably by reactivating the BLM complex once it generated free radicals. Cysteamine (positive) potentiated BLM action the most, glutathione (negative) potentiated this antibiotic the least, whereas cysteine had an intermediate effect compared with the other two.
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Laffon Blanca, Fernández-Bertólez Natalia, Costa Carla, Pásaro Eduardo, Valdiglesias Vanessa, Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing, Food and Chemical Toxicology, 102, 2017. Crossref
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Olsen Cathrine Elisabeth, Sellevold Simen, Theodossiou Theodossis, Patzke Sebastian, Berg Kristian, Impact of genotypic and phenotypic differences in sarcoma models on the outcome of photochemical internalization (PCI) of bleomycin, Photodiagnosis and Photodynamic Therapy, 20, 2017. Crossref