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Journal of Environmental Pathology, Toxicology and Oncology
インパクトファクター: 1.625 5年インパクトファクター: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2019030172
pages 313-327

Discrimination by RT-Nested PCR of Alpha-Lactalbumin Transcript Expression in Mammary Tumors

Ines Bessrour
Université de Tunis El Manar, Faculté des Sciences de Tunis, LR05ES05 Génétique, Immunologie et Pathologies Humaines, 2092, El Manar I Tunis, Tunisia
Soumaya Kouidhi
Université de Tunis El Manar, Faculté des Sciences de Tunis, LR05ES05 Génétique, Immunologie et Pathologies Humaines, 2092, El Manar I Tunis, Tunisia
Slim Belhassen
28, Rue Mosbah Jarbou El Manar 2. 2092 Tunis Tunisie
Amel Benammar Elgaaïed
Université de Tunis El Manar, Faculté des Sciences de Tunis, LR05ES05 Génétique, Immunologie et Pathologies Humaines, 2092, El Manar I Tunis, Tunisia

要約

Alpha-lactalbumin is a protein of milk expressed by mammary epithelial cells and by some breast tumors. The alpha-lactalbumin gene has two transcripts. The expression of transcript 2 has not yet been studied. The main objective of this paper was to establish the expression profile of alpha-lactalbumin at the mRNA level and to develop a technique discriminating between the two transcripts. The study was performed on 46 fresh mammary biopsies: 17 malignant tumors, 13 benign tumors, and 16 adjacent healthy tissues. We developed an RT-nested PCR to detect LALBA gene expression without distinction between transcripts. We also designed an RT-nested PCR that detects transcript 2. Both nested PCRs avoid amplification of potentially contaminating genomic DNA. We show that benign tumors tend to appear at a young age contrarily to malignant tumors appearing later with p value = 0.002. Moreover, LALBA transcript expression varies among tissues more important in benign (69%) than in malignant (35%) tumors with significant p value = 0.041. The intensity of the bands reflected differential expression between patients with concordant data between both RT-nested PCRs. We also performed a qualitative analysis according to histopathological parameters and molecular subtypes. The expression of transcript 2 appeared associated with the HER2+ subtype, while transcript 1 was associated with the luminal A and triple-negative subtypes. In conclusion, we designed specific and sensitive methods to detect LALBA transcripts. We show for the first time a differential expression of these transcripts, but we need to confirm their potential use as markers in breast cancer.

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