ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
Journal of Environmental Pathology, Toxicology and Oncology
インパクトファクター: 1.241 5年インパクトファクター: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2013006843
pages 91-99

Cellular Internalization and Mechanism of Cytotoxicity of 131I-Rituximab in Raji Cells

Chandan Kumar
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai, India
Badri Narain Pandey
Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India
Grace Samuel
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai, India
Meera Venkatesh
Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai, India

要約

Rituximab labeled with radioiodine (131I-rituximab) has a large potential to be employed for targeted therapy of non-Hodgkin's lymphoma. Studies of parameters such as cellular internalization, stability of 131I-rituximab bound to CD20 receptor of tumor cells, and the mechanism underlying cytotoxicity induced by 131I-rituximab will be useful for better clinical application. In this article we describe the efficacy of 131I-rituximab in CD20-expressing Raji cells. Rituximab labeled with 131I was purified on a PD-10 column and characterized using high-performance liquid chromatography and paper electrophoresis. Raji cells treated with 131I-rituximab (1.85 MBq for 2 hours) were washed then incubated. The culture medium collected from treated cells showed increased radioactivity over a longer period (>6 hours), probably due to the deiodination/degradation of 131I-rituximab. The tumor cells treated with 131I-rituximab showed time-dependent internalization of radioactivity, and at 12 hours the radioactivity was almost equally distributed in the membrane and cytoplasm. At 24 hours ~70% of the radioactivity was internalized. Cellular toxicity after 131I-rituximab treatment showed a time-dependent increase in toxicity as estimated by lactate dehydrogenase. Tumor cells treated with 131I-rituximab showed significantly higher toxicity and apoptosis compared with the those treated with the same concentration of unlabeled rituximab. The increased apoptotic death in cells treated with 131I-rituximab was associated with cleavage of poly ADP ribose polymerase and upregulation of p53 protein. This study provides a deeper understanding about the cellular internalization/stability of 131I-rituximab bound to the CD20 receptor and its efficacy in killing Raji cells.


Articles with similar content:

Immunotherapy of Colon Cancer Using Chimeric mAb 31.1
Critical Reviews™ in Immunology, Vol.18, 1998, issue 1-2
Tommy Wang, Myron Arlen, Al Tsang
5-Aminolevulinic Acid-Mediated Photodynamic Therapy on Hep-2 and MCF-7c3 Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 2
Haydee Fukuda, M. S. Lacelli, Maria Gabriela Alvarez, Alcira Batlle, Viviana Rivarola
Stimulation of Human Innate Immune Cells by Medicinal Mushroom Sclerotial Polysaccharides
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 3
Ka-Hing Wong, Peter Chi Keung Cheung, Connie K. M. Lai
Photochemoprotective Effect of Calluna vulgaris Extract on Skin Exposed to Multiple Doses of Ultraviolet B in SKH-1 Hairless Mice
Journal of Environmental Pathology, Toxicology and Oncology, Vol.31, 2012, issue 3
Elena Diana Olteanu, Marcela Achim, Pompei Bolfa, Laura Bolojan, Ion Dan Postescu, Adriana Muresan, Doina Daicoviciu, Laurian Vlase, Adriana Filip, Simona Clichici
Beneficial Outcomes of Kimchi Prepared with Amtak Baechu Cabbage and Salting in Brine Solution: Anticancer Effects in Pancreatic and Hepatic Cancer Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.37, 2018, issue 2
Kun-Young Park, Seung-Min Lee, Eui-Seong Park, Dong-Bok Park, Gyl-Hoon Song