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Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN 印刷: 1947-5764
ISSN オンライン: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2017019140
pages 339-347

Effect of Oxygen on Decontamination of Cumin Seeds by Atmospheric Pressure Dielectric Barrier Discharge Plasma

Soheila Abdi
Department of Physics, Safadasht Branch, Islamic Azad University, Tehran, Iran
Davoud Dorranian
Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran
Kyana Mohammadi
Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

要約

We studied the effect of oxygen on decontamination of cumin seeds by atmospheric pressure dielectric barrier discharge (DBD) plasma. A peak-to-peak 15-kV radio-frequency power supply at 17-kHz frequency was used to generate Ar and Ar-O2 plasmas between two circular electrodes. Electrodes were covered by mica plates with a 5-mm gap between each plate. Seeds were exposed to plasma until they were completely decontaminated. To determine total bacteria content, the seeds were cultured using a pour-plate method on a nutrient agar medium. To evaluate bacterial destruction, concentrations of double-stranded DNA and protein were determined spectrophotometrically. Results indicate that the surviving microorganism density decreased significantly after exposure of Ar and Ar-02 plasma in a time-dependent manner. Complete elimination of total bacteria was obtained after 20 and 40 min of exposure for Ar-O2 and Ar plasma, respectively. Our research shows that atmospheric pressure DBD plasma using Ar or Ar-O2 gas may be a suitable alternative method for decontamination of cumin seeds, without resulting in any detrimental effects on food quality of seeds.


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