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Plasma Medicine

年間 4 号発行

ISSN 印刷: 1947-5764

ISSN オンライン: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

Indexed in

Identification of the Molecular Basis of Non-thermal Plasma-Induced Changes in Human Keratinocytes

巻 3, 発行 1-2, 2013, pp. 15-25
DOI: 10.1615/PlasmaMed.2014008535
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要約

A comprehensive gene expression profiling was conducted to explore cellular effects after non-thermal plasma atmospheric pressure plasma treatment. We performed a high-content microarray comparison by assessing several categories of target probes in identical conditions of labeling, hybridization, and data analysis to compare specific gene expression profiles of human epithelial skin cells with their nontreated counterparts. For assessment of transcriptome changes, cell culture medium was plasma treated and applied to the HaCaT keratinocyte cell culture. We show that even this indirect argon plasma treatment performs as well as incubation time−dependent effects on gene expression. These effects range from cell proliferation and growth to the induction of cell death pathways. It is hypothesized that these effects are evoked through plasma-based formation of reactive oxygen and nitrogen species. Several significant biological pathways, such as oxidative stress, repair, and inflammation signaling, as well as >300 transcription factors (e.g., zinc finger or homebox) were identified. Our results contribute to a better understanding of plasmamediated changes in cells at the transcriptional level. In addition, plasma may serve as a promising biomedical tool for stimulation of skin cells with regard to wound healing.

によって引用された
  1. Haertel Beate, Woedtke Thomas von, Weltmann Klaus-Dieter, Lindequist Ulrike, Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing, Biomolecules & Therapeutics, 22, 6, 2014. Crossref

  2. Lendeckel Derik, Eymann Christine, Emicke Philipp, Daeschlein Georg, Darm Katrin, O’Neil Serena, Beule Achim G., von Woedtke Thomas, Völker Uwe, Weltmann Klaus-Dieter, Jünger Michael, Hosemann Werner, Scharf Christian, Proteomic Changes of Tissue-Tolerable Plasma Treated Airway Epithelial Cells and Their Relation to Wound Healing, BioMed Research International, 2015, 2015. Crossref

  3. Korolov Ihor, Fazekas Barbara, Széll Márta, Kemény Lajos, Kutasi Kinga, The effect of the plasma needle on the human keratinocytes related to the wound healing process, Journal of Physics D: Applied Physics, 49, 3, 2016. Crossref

  4. Hänsch Mareike A C, Mann Miriam, Weltmann Klaus-Dieter, von Woedtke Thomas, Analysis of antibacterial efficacy of plasma-treated sodium chloride solutions, Journal of Physics D: Applied Physics, 48, 45, 2015. Crossref

  5. Kobayashi Mime, Tokaji Hideto, Kumagai Shinya, Direct plasma irradiation affects expression of RNAs in cultured mammalian cells, Applied Physics Express, 9, 12, 2016. Crossref

  6. von Woedtke Thomas, Schmidt Anke, Bekeschus Sander, Wende Kristian, Wissenschaftliche Grundlagen, Stand und Perspektiven der Plasmamedizin, in Plasmamedizin, 2016. Crossref

  7. von Woedtke Thomas, Schmidt Anke, Bekeschus Sander, Wende Kristian, Introduction to Plasma Medicine, in Comprehensive Clinical Plasma Medicine, 2018. Crossref

  8. Schmidt Anke, Bekeschus Sander, Jablonowski Helena, Barton Annemarie, Weltmann Klaus-Dieter, Wende Kristian, Role of Ambient Gas Composition on Cold Physical Plasma-Elicited Cell Signaling in Keratinocytes, Biophysical Journal, 112, 11, 2017. Crossref

  9. Schmidt Anke, Dietrich Stephan, Steuer Anna, Weltmann Klaus-Dieter, von Woedtke Thomas, Masur Kai, Wende Kristian, Non-thermal Plasma Activates Human Keratinocytes by Stimulation of Antioxidant and Phase II Pathways, Journal of Biological Chemistry, 290, 11, 2015. Crossref

  10. Schmidt Anke, Bekeschus Sander, von Woedtke Thomas, Hasse Sybille, Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment, Clinical Plasma Medicine, 3, 1, 2015. Crossref

  11. Arndt Stephanie, Schmidt Anke, Karrer Sigrid, von Woedtke Thomas, Comparing two different plasma devices kINPen and Adtec SteriPlas regarding their molecular and cellular effects on wound healing, Clinical Plasma Medicine, 9, 2018. Crossref

  12. Marches Aurélie, Clement Emily, Albérola Géraldine, Rols Marie-Pierre, Cousty Sarah, Simon Michel, Merbahi Nofel, Cold Atmospheric Plasma Jet Treatment Improves Human Keratinocyte Migration and Wound Closure Capacity without Causing Cellular Oxidative Stress, International Journal of Molecular Sciences, 23, 18, 2022. Crossref

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