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Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN 印刷: 1947-5764
ISSN オンライン: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2014008450
pages 1-13

Differential Viability of Eight Human Blood Mononuclear Cell Subpopulations After Plasma Treatment

Sander Bekeschus
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Julia Kolata
Institute of Immunology and Transfusion Medicine, Department of Immunology, University of Greifswald, Greifswald, Germany
Anne Muller
ZIK plasmatis at Leibniz Institute for Plasma Science and Technology, Greifswald, Germany
Axel Kramer
Institute of Hygiene and Environmental Medicine, University Medicine Greifswald, 17475 Greifswald, Germany
Klaus-Dieter Weltmann
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Barbara Broker
Institute of Immunology and Transfusion Medicine, Department of Immunology, University of Greifswald, Greifswald, Germany
Kai Masur
Center for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology, Greifswald, Germany

要約

In plasma medicine, basic and translational research aids in future application of cold plasma sources in human diseases or disorders (e.g., chronic wounds). While most work has focussed on the interaction of skin cells with plasma, immune system cells have only been marginally examined. Their role is of major importance because they fulfill key regulatory parts in immune responses and modulate inflammation in all types of tissues. This work systematically investigates eight different subpopulations (monocytes and CD4+, CD8+, B, NK, NKT, TH17, and γδ T cells) of human peripheral blood mononuclear cells with regard to viability after 5, 20, or 60 s of plasma treatment. Twenty-four hours after exposure, viability differed between populations (23.1% CD4+ versus 41.9% γδ T cells after 60 s of exposure) as revealed by flow cytometry. Cellular activation before plasma treatment increased survival in all subpopulations tested (26.8% in nonstimulated versus 50.0% in stimulated CD8+ T cells after 60 s of exposure). All lymphocyte subpopulations showed significantly (P < 0.05) lower survival rates compared to monocytes (35.9% for B cells versus 82.5% for monocytes after 60 s of exposure) but not compared to each other, hallmarking two intrinsically different coping types of cells regarding plasma cytotoxicity.