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Plasma Medicine

年間 4 号発行

ISSN 印刷: 1947-5764

ISSN オンライン: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Reactive Oxygen Species and Intracellular Ca2+ Contribution to Micro-Discharge Plasma Gene Transfection

巻 7, 発行 4, 2017, pp. 321-332
DOI: 10.1615/PlasmaMed.2018019510
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要約

We investigated on the mechanism of plasma gene transfection. It is suggested that reactive oxygen species (ROS) directly or indirectly acts on endocytosis for gene transfection. The intracellular Ca2+ concentration was increased by plasma irradiation, and it was confirmed there is some kind of relationship between ROS and release of the intracellular Ca2+. It is clear that ROS are the most important chemical factors in plasma gene transfection and that Ca2+ is not a dominant factor but a subsidiary or consequential factor. Our studies also confirmed that the transfer mechanism for low molecular weight substances, such as YOYO-1, is different from the mechanism for molecules of higher weight, such as plasmid DNA. In the case of larger molecules, ROS play an important role by inducing endocytosis in plasma gene transfection. But in the case of smaller molecules, neither ROS nor ROS-dependent endocytosis is dominant. Some kind of "endocytosis-independent and ROS-free" transfer processes may exist for smaller molecules to be transferred into cells by plasm irradiation. To discuss the mechanism of micro-plasma gene transfection, it is important to note that the transfer mechanism is different for substances of different molecular sizes.

によって引用された
  1. Hiramatsu Tatsuya, Hirashige Hiroko, Kido Yugo, Satoh Susumu, Jinno Masafumi, Importance of collision frequency in the molecular size dependency of gene transfer efficiency in the surface discharge method, Japanese Journal of Applied Physics, 58, SE, 2019. Crossref

  2. Przekora Agata, Pawlat Joanna, Terebun Piotr, Duday David, Canal Cristina, Hermans Sophie, Audemar Maïté, Labay Cédric, Thomann Jean-Sébastien, Ginalska Grazyna, The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro, Journal of Physics D: Applied Physics, 52, 27, 2019. Crossref

  3. Kido Yugo, Motomura Hideki, Ikeda Yoshihisa, Satoh Susumu, Jinno Masafumi, Yousfi Mohammed, Clarification of electrical current importance in plasma gene transfection by equivalent circuit analysis, PLOS ONE, 16, 1, 2021. Crossref

  4. Jinno Masafumi, Satoh Susumu, Ikeda Yoshihisa, Motomura Hideki, The new technology of molecular and gene introduction method using discharge plasma: plasma brings features of random genome integration-free and damage-free to cells, genomic-DNA and external introducing molecules, Japanese Journal of Applied Physics, 60, 3, 2021. Crossref

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