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Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN 印刷: 1947-5764
ISSN オンライン: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2014011874
pages 17-28

Plasma-based Stimulation of Biotechnological Processes in Ganoderma lucidum Mycelia as Example for a Eukaryotic Organism

Beate Haertel
Institute of Pharmacy, Pharmaceutical Biology, University of Greifswald Greifswald, Germany; Leibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany
Christian Backer
Institute of Pharmacy, Pharmaceutical Biology, University of Greifswald Greifswald, Germany; Leibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany
Christian Schulze
Institute of Pharmacy, Department of Pharmaceutical Biology, University of Greifswald, 17487 Greifswald, Germany
Anja Funke
Institute of Pharmacy, Pharmaceutical Biology, University of Greifswald Greifswald, Germany
Martina Wurster
Institute of Pharmacy, Pharmaceutical Biology, University of Greifswald Greifswald, Germany
Thomas von Woedtke
Leibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany
Ulrike Lindequist
Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 17, D-17489 Greifswald, Germany

要約

In biotechnological processes, high-yielding production strains are the condition for economic production of biomass and desired metabolites. Plasma with its components like UV radiation and highly reactive radicals could possibly generate and improve new production strains. Indeed, plasma was able to generate high-yielding bacterial strains. A candidate for eukaryotic cells for improving metabolite production is the medicinal mushroom Ganoderma lucidum used for prevention and treatment of many different diseases. Mycelia and fruit bodies contain more than 150 structurally different triterpens. The aim of our investigation was to check whether plasma influences growth and productivity of the fungus. Mycelial cultures were exposed to plasma for 2−20 min by using three different plasma sources: the plasma jet klNPen 09 and two dielectric barrier discharge plasma sources (surface-DBD, volume-DBD). Yield of biomass and extracts were determined. HPLC fingerprints of organic extracts were recorded. Content of triterpens, ganoderic acid A, ergosterol, and β-1-3 D-glucan was determined. Volume-DBD increased extract yield and β-1-3 D-glucan content, while whole triterpenoid content was increased by the plasma jet. Both dielectric barrier discharge plasma sources led to elevated ergosterol contents. The effects seemed to be related to energy input by the plasma sources. In conclusion, plasma could be principally useful for optimization of biotechnological processes not for only prokaryotic but also eukaryotic cells.