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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN 印刷: 2155-014X
ISSN オンライン: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v1.i1.10
pages 1-7

Effect of β-amyloid Peptide on Plasmalemmal Calcium Channels in Cultured Hippocampal Neurons

Tatyana Yu. Korol
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Elena P. Kostyuk
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Platon G. Kostyuk
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv

要約

Anomalous accumulation of β-amyloid peptide in cerebral neurons plays central role in pathogenesis of Alzheimer’s disease (AD). One of the essential pathogens at AD is dis- turbance of calcium homeostasis in neurons of central nervous system. It was determined in this work that 24-hour incubation of hippocampal cell culture with β-amyloid peptide caused more than twofold elevation of basal calcium concentration relatively to control value ((153.4 ± 11.5) and (71.7 ± 5.4) nM respectively; Р < 0.05, n = 7). Using whole cell patch-clamp technique it was detected that calcium current density in β-amyloid-treated cells was 70% higher (P < 0.05, n = 12) than in control ones. Obtained data broaden our comprehension of disturbance of molecular mechanisms of calcium homeostasis in neurons at AD particularly mechanisms of elevation of basal calcium concentration by means of enhancement of calcium influx through plasmalemmal voltage-gated calcium channels.