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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN 印刷: 2155-014X
ISSN オンライン: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v1.i1.20
pages 9-16

Changes in Synaptic Plasticity due to Blockage of N-type Ca2+ Channels in Cultured Hippocampal Neurons

Oksana P. Kolesnyk-Mizerna
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Svetlana A. Fedulova
O. O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Mycola S. Veselovsky
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

要約

However, it is not known whether presynaptic N-type Ca2+ channels contribute to short-term synaptic plasticity (STP) mediated by GABA release at inhibitory synapses of cultured hippocampal neurons. We studied the sensitivity of GABAergic paired pulse depression (PPD) as a common form of STP to selective N-type high-voltage-activated Ca2+ channels blocker omega-conotoxin (ω-CgTx). Evoked inhibitory postsynaptic currents (eIPSCs) were studied using patch-clamp technique in whole-cell configuration in postsynaptic neuron and local extracellular paired pulse stimulation of single presynaptic axon by rectangular pulse with 0.4 ms duration, the impulse interval in pair was 150 ms. ω-CgTx (200 nM; 1 μM) in a dose-dependent manner irreversibly reduced the amplitude of paired eIPSCs by 25−49% and decreased PPD by 11−22% as compared with control. These results confirm that N-type Ca2+ channels are highly involved in inhibitory synaptic transmission and short-term synaptic plasticity in cultured hippocampal neurons.