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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN 印刷: 2155-014X
ISSN オンライン: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v1.i1.40
pages 25-34

Migration and Differentiation of Fetal Neural Progenitor Cells in the Brain of Ischemic Animals

O. M. Tsupykov
Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv
Tetyana A. Pivneva
Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv; State Key Laboratory of Molecular and Cellular Biology, Bogomolets Institute of Physiology, Kyiv, Ukraine
A. O. Poddubna
Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv
Vitaliy Kyryk
State Institute of Genetic and Regenerative Medicine, AMN of Ukraine, Kyiv
O. V. Kuchuk
Institute of genetic and regenerative medicine AMN of Ukraine, Kyiv
Gennadi M. Butenko
Institute of Gerontology; and Institute of genetic and regenerative medicine AMN of Ukraine, Kyiv
Galina G. Skibo
A. A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

要約

The migration, integration and differentiation of fetal neural progenitor cells (NPCs) in the ischemic brain have been studied. Nowadays there is no effective therapy to promote recovery following ischemic insult. Neural stem cells have been proposed as a potential source of new cells to replace those lost due to central nervous system injury, as well as a source of trophic molecules to minimize damage and promote recovery. In our study the ischemic insult in FVB line mice was modulated by occlusion of both carotid arteries during 20 min. Day after occlusion NPCs from GFP-transgenic 12.5 dpc embryos were suboccipitally transplanted to the ischemic brain. The migration and differentiation of GFP-positive NPCs in the recipient tissue were observed at different time points after their transplantation by immu- nohistochemical approaches using confocal scanning microscopy. It has been shown that GFP-positive NPCs survived, migrated and differentiated to the mature neurons and glial cells in CA1 area of hippocampus of ischemic animals.