ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i4.10
pages 331-345

Study on the Analytical Method for Water-soluble Crude Polysaccharides in Culinary-Medicinal Mushrooms

Shu-Fang Lu
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.
Shu-Chi Tsai
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.
Hsiao-Chen Lin
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.
Weiguang Fu
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.

要約

Size exclusion high performance liquid chromatography (SE-HPLC) was used to analyze water extracts of edible medicinal mushrooms in this research. Fingerprint profiling of polysaccharides in edible medicinal mushrooms was utilized as the primary identification tool for qualitative analysis. Furthermore, this study successfully improves or corrects the overestimation problem in quantifying functional polysaccharide contents in phenol-sulfuric acid method. The comparison of 3 different pre-treatment methods including SE-HPLC fractionation, ultra-filtration (UF) fractionation and alcohol precipitation shows that SE-HPLC fractionation method not only can recover the loss of polysaccharides in UF or alcohol precipitation methods, but also is able to fully retain water-soluble crude polysaccharides with molecular weight > 10 kD, which are commonly regarded as functional components in medicinal mushrooms. Therefore, the combination of SE-HPLC fractionation and phenol-sulfuric acid quantification method is more accurate and suitable for determination of water-soluble crude polysaccharide in edible medicinal mushrooms.


Articles with similar content:

Polysaccharides and Antioxidants from Culinary-Medicinal White Button Mushroom, Agaricus bisporus (Agaricomycetes), Waste Biomass
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 8
Jasenka Piljac-Zegarac, Vlatka Petravić-Tominac, Senka Djakovic, Vesna Zechner-Krpan, Sinisa Srecec, Aleksandra Vojvodic Cebin, Omoanghe S. Isikhuemhen
Antioxidant and Immunomodulating Activities of Exo-and Endopolysaccharide Fractions from Submerged Mycelia Cultures of Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 3
Karthik Marripudi, Cheang Khoo, Sundar Rao Koyyalamudi, Jong Pil Park, Jin Hee Kim, Sang-Chul Jeong, Chi-Hyun Song, J. Margaret Hughes, Trevor Bailey
Hypoglycemic, Interferonogenous, and Immunomodulatory Activity of Tremellastin from the Submerged Culture of Tremella mesenterica Retz.: Fr. (Heterobasidiomycetes)
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 3
Solomon P. Wasser, Kok- Kheng Tan, Vladimir I. Elisashvili
Antioxidative and Inhibitory Effects of the Fruiting Body of Black Lingzhi Mushroom, Amauroderma rugosum (Agaricomycetes), on LDL Oxidation and HMG-CoA Reductase Activity
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 9
Norhaniza Aminudin, Noorlidah Abdullah, Chan Kam Seng
Effect of Sasa veitchii Extract on Immunostimulating Activity of β-glucan (SCG) from Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Mia Yoshida, Naohito Ohno, Toshie H. Hida, Natsuko Akachi