ライブラリ登録: Guest
International Journal of Medicinal Mushrooms

年間 12 号発行

ISSN 印刷: 1521-9437

ISSN オンライン: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

Indexed in

Protoplast Fusion between Pleurotus ostreatus (Jacq.:Fr.) P. Kumm. and P. citrinopileatus Singer

巻 7, 発行 3, 2005, 397 pages
DOI: 10.1615/IntJMedMushrooms.v7.i3.440
Get accessDownload

要約

Protoplast fusion has been used as a method to create mushroom hybrids, especially when using conventional methods cannot achieve this result. Pleurotus ostreatus and P. citrinopileatus, along with many other species of the genus Pleurotus, are widely industrially cultivated in many parts of the world, including Thailand.
Protoplasts fusion between P. ostreatus and P. citrinopileatus in this experiment was carried out by growing 4-day-old monokaryotic mycelia derived from a single spore isolate of each species on malt extract broth. The mycelia were agitated at 100 rpm for 2 hours with 9 mg membrane-filtration sterilized Lysing Enzyme (Sigma L-1412) in 1 mL osmotic stabilizer (0.6 M MgSO4(7H2O) in 0.05 M sodium maleate buffer, pH 5). The freshly prepared protoplasts were then mixed and incubated in 40% PEG (polyethylene glycol 6,000)/0.05 M CaCl2(2H2O) for 20 minutes at room temperature. All protoplasts were regenerated on regeneration medium containing 30 g agar, 20 g malt extract, 20 g glucose, 1 g peptone, and 1000 mL 0.6 M sucrose for 7−12 days. There were 487 regenerated colonies detected, but only three of them were selected as fusants by posessing clamp connections on their mycelia. The fusants (Fu1, Fu2, and Fu3) were proved to be “hybrids” of P. ostreatus and P. citrinopileatus as the experiment progressed.
The mycelia of the fusants were significantly faster in growth and larger in size than the parental strains, which were relevant to the theories that fusants, which are dikaryotic (n + n), grow faster (Toyomatsu and Mori, 1987) and have larger hyphae (Abe et al., 1982) than their monokaryotic (n) parental strains. The experiments were performed by subculturing the mycelia of each fusant and of the two parents on MEA plates and incubating for 9 days at room temperature followed by determining the diameters of each colony for at least 10 replications as the mycelial growth and measuring the diameters of the mycelia microscopically using a calibrated eyepiece micrometer for 100 replications as the hyphal size.
The fusants showed esterase and malate dehy-drogenase isozyme bands common to those of their parents when esterase, malate dehydrogenase, and alcohol dehydrogenase were studied as the method modified from that of Pasteur et al. (1988).
The fruiting bodies of Fu1, which was the only fusant successful in fruiting on sawdust plastic bags, showed recombined characteristics of the parents. Its fruiting bodies were yellow in color, which was similar to P. citrinopileatus, but its spore print was creamish color, which was similar to P. ostreatus. P. ostreatus has a creamish color both in its fruiting bodies and spore prints, while P. citrinopileatus has golden yellowish color in its fruiting bodies but is pinkish grey in spore sprints.

によって引用された
  1. He Peixin, Yu Miao, Wang Ke, Cai Yingli, Li Bin, Liu Wei, Interspecific hybridization between cultivated morels Morchella importuna and Morchella sextelata by PEG-induced double inactivated protoplast fusion, World Journal of Microbiology and Biotechnology, 36, 4, 2020. Crossref

  2. Zhang Zichen, Shah Aabid Manzoor, Mohamed Hassan, Zhang Yao, Sadaqat Beenish, Tsiklauri Nino, Sadunishvili Tinatin, Song Yuanda, Improved laccase production in Pleurotus djamor RP by atmospheric and room temperature plasma (ARTP) mutagenesis, Electronic Journal of Biotechnology, 58, 2022. Crossref

Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集 価格及び購読のポリシー Begell House 連絡先 Language English 中文 Русский Português German French Spain